In vitro stimulation of bovine peripheral blood lymphocytes: effect of short-term storage of blood prior to lymphocyte culture.

D. R. Senogles, C. C. Muscoplat, J. M. Kaneene, C. O. Thoen, D. W. Johnson

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Storage of peripheral blood from Mycobacterium bovis-sensitized cattle from 1 to 48 hours at 4, 22, and 37 C was shown not to alter markedly the lymphocyte blastogenic response to M bovis-purified protein derivative. Concanavalin A-induced lymphocyte mitogenic responses were unaffected by storage of blood for 1, 24, or 48 hours at 22 C and 37 C; however, storage of blood for 48 hours at 4 C significantly lowered (P less than 0.05) mitogenic responses to concanavalin A, as compared with responses to blood kept at 22 C. Mononuclear cell recovery from stored blood at all temperatures was markedly less than that from freshly drawn blood samples. Cell recoveries were most affected by storage of blood at 4 C and 37 C.

Original languageEnglish (US)
Pages (from-to)341-344
Number of pages4
JournalAmerican journal of veterinary research
Volume39
Issue number2
StatePublished - Feb 1 1978

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