TY - JOUR
T1 - In vitro susceptibility of cytomegalovirus isolates from immunocompromised patients to acyclovir and ganciclovir
AU - Cole, Nancy L.
AU - Balfour, Henry H.
N1 - Funding Information:
Supported by Grant AM 13083 from the NIH, and grants from the Minnesota Medical Foundation and Burroughs Wellcome Co.
PY - 1987/3
Y1 - 1987/3
N2 - Fifty-four isolates of cytomegalovirus (CMV) from 25 immunocompromised patients with CMV infections were examined in a plaque reduction assay to determine in vitro susceptibilities to both acyclovir and ganciclovir. Isolates were approximately 25-fold more sensitive to ganciclovir than to acyclovir. The mean ± SD ID50 for all isolates to acyclovir was 63.1 ± 30.2 μM, (median, 52.3 μM; range, 16.7-146.4 μM). The mean ID50 for all isolates to ganciclovir was 2.50 ± 1.27 μM, (median, 2.15 μM; range, 0.65-7.11 μM). Exposure to acyclovir or ganciclovir for periods of 2-5 wk did not alter the mean susceptibility of clinical isolates. However, one CMV strain isolated from a renal transplant patient after 14 days of acyclovir therapy displayed the lowest susceptibility of all strains tested (acyclovir 146.4 μM; ganciclovir 7.11 μM) and may represent selection of a resistant virus population.
AB - Fifty-four isolates of cytomegalovirus (CMV) from 25 immunocompromised patients with CMV infections were examined in a plaque reduction assay to determine in vitro susceptibilities to both acyclovir and ganciclovir. Isolates were approximately 25-fold more sensitive to ganciclovir than to acyclovir. The mean ± SD ID50 for all isolates to acyclovir was 63.1 ± 30.2 μM, (median, 52.3 μM; range, 16.7-146.4 μM). The mean ID50 for all isolates to ganciclovir was 2.50 ± 1.27 μM, (median, 2.15 μM; range, 0.65-7.11 μM). Exposure to acyclovir or ganciclovir for periods of 2-5 wk did not alter the mean susceptibility of clinical isolates. However, one CMV strain isolated from a renal transplant patient after 14 days of acyclovir therapy displayed the lowest susceptibility of all strains tested (acyclovir 146.4 μM; ganciclovir 7.11 μM) and may represent selection of a resistant virus population.
UR - http://www.scopus.com/inward/record.url?scp=0023281112&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023281112&partnerID=8YFLogxK
U2 - 10.1016/0732-8893(87)90020-4
DO - 10.1016/0732-8893(87)90020-4
M3 - Article
C2 - 3032503
AN - SCOPUS:0023281112
SN - 0732-8893
VL - 6
SP - 255
EP - 261
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 3
ER -