In vivo bioluminescence tumor imaging of RGD peptide-modified adenoviral vector encoding firefly luciferase reporter gene

Gang Niu; Zhengming Xiong; Zhen Cheng; Weibo Cai; Sanjiv S. Gambhir; Lei Xing; Xiaoyuan Chen

doi:10.1007/s11307-007-0079-2

In vivo bioluminescence tumor imaging of RGD peptide-modified adenoviral vector encoding firefly luciferase reporter gene

Gang Niu, Zhengming Xiong, Zhen Cheng, Weibo Cai, Sanjiv S. Gambhir, Lei Xing, Xiaoyuan Chen

Pediatrics - Hematology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Purpose: The goal of this study is to demonstrate the feasibility of chemically modified human adenovirus (Ad) vectors for tumor retargeting. Procedures: E1- and E3-deleted Ad vectors carrying firefly luciferase reporter gene under cytomegalovirus promoter (AdLuc) was surface-modified with cyclic arginine-glycine-aspartic acid (RGD) peptides through a bifunctional poly(ethyleneglycol) linker (RGD-PEG-AdLuc) for integrin α_vβ₃ specific delivery. The Coxsackie and adenovirus viral receptor (CAR) and integrin α_vβ₃ expression in various tumor cell lines was determined by reverse transcriptase PCR and fluorescence-activated cell sorting. Bioluminescence imaging was performed in vitro and in vivo to evaluate RGD-modified AdLuc infectivity. Results: RGD-PEG-AdLuc abrogated the native CAR tropism and exhibited significantly enhanced transduction efficiency of integrin-positive tumors than AdLuc through intravenous administration. Conclusion: This approach provides a robust platform for site-specific gene delivery and noninvasive monitoring of the transgene delivery efficacy and homing.

Original language	English (US)
Pages (from-to)	126-134
Number of pages	9
Journal	Molecular Imaging and Biology
Volume	9
Issue number	3
DOIs	https://doi.org/10.1007/s11307-007-0079-2
State	Published - May 2007

Bibliographical note

Funding Information:
Acknowledgments. This work was supported, in part, by National Institute of Biomedical Imaging and Bioengineering (R21 EB001785), National Cancer Institute (R21 CA102123, P50 CA114747, U54 CA119367, and R24 CA93862), Department of Defense (W81XWH-04-1-0697, W81XWH-06-1-0665, W81XWH-06-1-0042, and DAMD17-03-1-0143), and a Benedict Cassen Postdoctoral Fellowship from the Education and Research Foundation of the Society of Nuclear Medicine (to WC). GN and ZX contributed equally to this work.

Keywords

Adenovirus
Bioluminescence imaging
Firefly luciferase
Integrin αβ
RGD
Reporter gene

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1007/s11307-007-0079-2

OpenUrl availability

Full text

Cite this

@article{0e2fd184a3054161ba9dfb6711cec56a,

title = "In vivo bioluminescence tumor imaging of RGD peptide-modified adenoviral vector encoding firefly luciferase reporter gene",

abstract = "Purpose: The goal of this study is to demonstrate the feasibility of chemically modified human adenovirus (Ad) vectors for tumor retargeting. Procedures: E1- and E3-deleted Ad vectors carrying firefly luciferase reporter gene under cytomegalovirus promoter (AdLuc) was surface-modified with cyclic arginine-glycine-aspartic acid (RGD) peptides through a bifunctional poly(ethyleneglycol) linker (RGD-PEG-AdLuc) for integrin αvβ3 specific delivery. The Coxsackie and adenovirus viral receptor (CAR) and integrin αvβ3 expression in various tumor cell lines was determined by reverse transcriptase PCR and fluorescence-activated cell sorting. Bioluminescence imaging was performed in vitro and in vivo to evaluate RGD-modified AdLuc infectivity. Results: RGD-PEG-AdLuc abrogated the native CAR tropism and exhibited significantly enhanced transduction efficiency of integrin-positive tumors than AdLuc through intravenous administration. Conclusion: This approach provides a robust platform for site-specific gene delivery and noninvasive monitoring of the transgene delivery efficacy and homing.",

keywords = "Adenovirus, Bioluminescence imaging, Firefly luciferase, Integrin αβ, RGD, Reporter gene",

author = "Gang Niu and Zhengming Xiong and Zhen Cheng and Weibo Cai and Gambhir, {Sanjiv S.} and Lei Xing and Xiaoyuan Chen",

note = "Funding Information: Acknowledgments. This work was supported, in part, by National Institute of Biomedical Imaging and Bioengineering (R21 EB001785), National Cancer Institute (R21 CA102123, P50 CA114747, U54 CA119367, and R24 CA93862), Department of Defense (W81XWH-04-1-0697, W81XWH-06-1-0665, W81XWH-06-1-0042, and DAMD17-03-1-0143), and a Benedict Cassen Postdoctoral Fellowship from the Education and Research Foundation of the Society of Nuclear Medicine (to WC). GN and ZX contributed equally to this work.",

year = "2007",

month = may,

doi = "10.1007/s11307-007-0079-2",

language = "English (US)",

volume = "9",

pages = "126--134",

journal = "Molecular Imaging and Biology",

issn = "1536-1632",

publisher = "Springer New York",

number = "3",

}

TY - JOUR

T1 - In vivo bioluminescence tumor imaging of RGD peptide-modified adenoviral vector encoding firefly luciferase reporter gene

AU - Niu, Gang

AU - Xiong, Zhengming

AU - Cheng, Zhen

AU - Cai, Weibo

AU - Gambhir, Sanjiv S.

AU - Xing, Lei

AU - Chen, Xiaoyuan

N1 - Funding Information: Acknowledgments. This work was supported, in part, by National Institute of Biomedical Imaging and Bioengineering (R21 EB001785), National Cancer Institute (R21 CA102123, P50 CA114747, U54 CA119367, and R24 CA93862), Department of Defense (W81XWH-04-1-0697, W81XWH-06-1-0665, W81XWH-06-1-0042, and DAMD17-03-1-0143), and a Benedict Cassen Postdoctoral Fellowship from the Education and Research Foundation of the Society of Nuclear Medicine (to WC). GN and ZX contributed equally to this work.

PY - 2007/5

Y1 - 2007/5

N2 - Purpose: The goal of this study is to demonstrate the feasibility of chemically modified human adenovirus (Ad) vectors for tumor retargeting. Procedures: E1- and E3-deleted Ad vectors carrying firefly luciferase reporter gene under cytomegalovirus promoter (AdLuc) was surface-modified with cyclic arginine-glycine-aspartic acid (RGD) peptides through a bifunctional poly(ethyleneglycol) linker (RGD-PEG-AdLuc) for integrin αvβ3 specific delivery. The Coxsackie and adenovirus viral receptor (CAR) and integrin αvβ3 expression in various tumor cell lines was determined by reverse transcriptase PCR and fluorescence-activated cell sorting. Bioluminescence imaging was performed in vitro and in vivo to evaluate RGD-modified AdLuc infectivity. Results: RGD-PEG-AdLuc abrogated the native CAR tropism and exhibited significantly enhanced transduction efficiency of integrin-positive tumors than AdLuc through intravenous administration. Conclusion: This approach provides a robust platform for site-specific gene delivery and noninvasive monitoring of the transgene delivery efficacy and homing.

AB - Purpose: The goal of this study is to demonstrate the feasibility of chemically modified human adenovirus (Ad) vectors for tumor retargeting. Procedures: E1- and E3-deleted Ad vectors carrying firefly luciferase reporter gene under cytomegalovirus promoter (AdLuc) was surface-modified with cyclic arginine-glycine-aspartic acid (RGD) peptides through a bifunctional poly(ethyleneglycol) linker (RGD-PEG-AdLuc) for integrin αvβ3 specific delivery. The Coxsackie and adenovirus viral receptor (CAR) and integrin αvβ3 expression in various tumor cell lines was determined by reverse transcriptase PCR and fluorescence-activated cell sorting. Bioluminescence imaging was performed in vitro and in vivo to evaluate RGD-modified AdLuc infectivity. Results: RGD-PEG-AdLuc abrogated the native CAR tropism and exhibited significantly enhanced transduction efficiency of integrin-positive tumors than AdLuc through intravenous administration. Conclusion: This approach provides a robust platform for site-specific gene delivery and noninvasive monitoring of the transgene delivery efficacy and homing.

KW - Adenovirus

KW - Bioluminescence imaging

KW - Firefly luciferase

KW - Integrin αβ

KW - RGD

KW - Reporter gene

UR - http://www.scopus.com/inward/record.url?scp=34248201700&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248201700&partnerID=8YFLogxK

U2 - 10.1007/s11307-007-0079-2

DO - 10.1007/s11307-007-0079-2

M3 - Article

C2 - 17297551

AN - SCOPUS:34248201700

SN - 1536-1632

VL - 9

SP - 126

EP - 134

JO - Molecular Imaging and Biology

JF - Molecular Imaging and Biology

IS - 3

ER -

In vivo bioluminescence tumor imaging of RGD peptide-modified adenoviral vector encoding firefly luciferase reporter gene

Abstract

Bibliographical note

Keywords

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this