The major exocellular glycopeptide (peptidophosphogalactomannan) produced by Penicillium charlesii first appears in the culture filtrate when the growth medium is nearly depleted of NH4+. The extent of incorporation of exogenously supplied radioactive precursors (d-[U-14C] glucose, l-[U-14C]threonine and NaH232PO4) into peptidophosphogalactomannan suggests that approximately 20% of the total quantity of peptidophosphogalactomannan is assembled from constituents taken from the growth medium before NH4+ starvation and that the remainder is assembled from constituents in the medium during NH4+ starvation. In the absence of NH4+, an increase in dry weight continues until the medium is depleted of glucose. However, peptidophosphogalactomannan accumulation proceeds until after glucose is depleted and growth is halted. These data suggest that peptidophosphogalactomannan is a product of cellular turnover.
- Penicillium charlesii
- in vivo