In vivo 13C spectroscopy in the rat brain using hyperpolarized [1-13C]pyruvate and [2-13C]pyruvate

Małgorzata Marjańska; Isabelle Iltis; Alexander A. Shestov; Dinesh K. Deelchand; Christopher Nelson; Kâmil Uurbil; Pierre Gilles Henry

doi:10.1016/j.jmr.2010.07.006

In vivo ¹³C spectroscopy in the rat brain using hyperpolarized [1-¹³C]pyruvate and [2-¹³C]pyruvate

Małgorzata Marjańska, Isabelle Iltis, Alexander A. Shestov, Dinesh K. Deelchand, Christopher Nelson, Kâmil Uurbil, Pierre Gilles Henry

Center for Magnetic Resonance Research

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

The low sensitivity of ¹³C spectroscopy can be enhanced using dynamic nuclear polarization. Detection of hyperpolarized [1- ¹³C]pyruvate and its metabolic products has been reported in kidney, liver, and muscle. In this work, the feasibility of measuring ¹³C signals of hyperpolarized ¹³C metabolic products in the rat brain in vivo following the injection of hyperpolarized [1-¹³C]pyruvate and [2-¹³C]pyruvate is investigated. Injection of [2-¹³C] pyruvate led to the detection of [2-¹³C]lactate, but no other downstream metabolites such as TCA cycle intermediates were detected. Injection of [1-¹³C]pyruvate enabled the detection of both [1- ¹³C]lactate and [¹³C]bicarbonate. A metabolic model was used to fit the hyperpolarized ¹³C time courses obtained during infusion of [1-¹³C]pyruvate and to determine the values of V _PDH and V_LDH.

Original language	English (US)
Pages (from-to)	210-218
Number of pages	9
Journal	Journal of Magnetic Resonance
Volume	206
Issue number	2
DOIs	https://doi.org/10.1016/j.jmr.2010.07.006
State	Published - Oct 2010

Bibliographical note

Funding Information:
The authors thank Manda Vollmers, Dee Koski, and William Mander from Oxford Instruments Molecular Biotools for technical support, Michael G. Garwood for helpful discussion about adiabatic pulses, and Josef Granwehr and Jamie D. Walls for comments about the paper. This work was supported by BTRR – P41 RR008079, P30 NS057091, RO1-NS38672, and the W.M. Keck Foundation. The high-resolution NMR facility at the University of Minnesota is supported with funds from the NSF (BIR-961477), the University of Minnesota Medical School, and the Minnesota Medical Foundation.

Keywords

Bicarbonate
DNP
Lactate
Pyruvate
Time courses

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title = "In vivo 13C spectroscopy in the rat brain using hyperpolarized [1-13C]pyruvate and [2-13C]pyruvate",

abstract = "The low sensitivity of 13C spectroscopy can be enhanced using dynamic nuclear polarization. Detection of hyperpolarized [1- 13C]pyruvate and its metabolic products has been reported in kidney, liver, and muscle. In this work, the feasibility of measuring 13C signals of hyperpolarized 13C metabolic products in the rat brain in vivo following the injection of hyperpolarized [1-13C]pyruvate and [2-13C]pyruvate is investigated. Injection of [2-13C] pyruvate led to the detection of [2-13C]lactate, but no other downstream metabolites such as TCA cycle intermediates were detected. Injection of [1-13C]pyruvate enabled the detection of both [1- 13C]lactate and [13C]bicarbonate. A metabolic model was used to fit the hyperpolarized 13C time courses obtained during infusion of [1-13C]pyruvate and to determine the values of V PDH and VLDH.",

keywords = "Bicarbonate, DNP, Lactate, Pyruvate, Time courses",

author = "Ma{\l}gorzata Marja{\'n}ska and Isabelle Iltis and Shestov, {Alexander A.} and Deelchand, {Dinesh K.} and Christopher Nelson and K{\^a}mil Uurbil and Henry, {Pierre Gilles}",

note = "Funding Information: The authors thank Manda Vollmers, Dee Koski, and William Mander from Oxford Instruments Molecular Biotools for technical support, Michael G. Garwood for helpful discussion about adiabatic pulses, and Josef Granwehr and Jamie D. Walls for comments about the paper. This work was supported by BTRR – P41 RR008079, P30 NS057091, RO1-NS38672, and the W.M. Keck Foundation. The high-resolution NMR facility at the University of Minnesota is supported with funds from the NSF (BIR-961477), the University of Minnesota Medical School, and the Minnesota Medical Foundation.",

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AU - Deelchand, Dinesh K.

AU - Nelson, Christopher

AU - Uurbil, Kâmil

AU - Henry, Pierre Gilles

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