Abstract
Glycosaminoglycans (GAGs) have been implicated in many functions of human blood cells. Normal human monocytes grown on plastic culture dishes for five days display macrophage-like morphology and functional characteristics concurrent with the appearance of a 4,6-disulfated chondroitin. This process has been regarded as in vitro differentiation. HL-60 human promyelocytic leukemia cells produce chondroitin 4-sulfate, which is the major GAG constitutent of normal granuloyctes and monocytes. These cells can be induced by phorbol myristate acetate (PMA) to develop many macrophage-like characteristics. This study investigates GAG production by HLA-60 cells after prolonged exposure to PMA. Disulfated chondroitin molecules were not detected. This biochemical abnormality may be an important factor in the functional deficiencies of these induced cells.
Original language | English (US) |
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Pages (from-to) | 672-675 |
Number of pages | 4 |
Journal | Experimental Hematology |
Volume | 14 |
Issue number | 7 |
State | Published - 1986 |
Externally published | Yes |