TY - JOUR
T1 - Independence of β-adrenergic stimulation of renin release on renin synthesis
AU - Katz, S. A.
AU - Malvin, R. L.
PY - 1982
Y1 - 1982
N2 - Rat renal cortical slices incubated in vitro released 7.0 ± 0.5% (mean ± SE, n = 30) of their total renin content into the incubation medium in 1 h. Isoproterenol (10-6 M), a β-adrenergic agonist, caused a 75 ± 17% (n = 7) increase in renin release from the cortical slices. Treatment of the cortical slices with either cycloheximide or puromycin caused a significant 96.2 ± 0.34 (n = 8) or 98.5 ± 0.3% (n = 5), respectively, inhibition of protein synthesis. After protein synthesis and presumably renin synthesis was blocked with either cycloheximide or puromycin, isoproterenol was still able to increase significantly renin release from the cortical slices despite almost total blockade of protein synthesis. We conclude that a storage pool of renin may exist, since 1) only 7.0 ± 0.5% of the cortical slice renin content is released each hour, and 2) isoproterenol stimulation of renin release is not acutely dependent on renin synthesis. It is hypothesized that β-adrenergic stimulation of renin release is elicited from a storage pool of previously synthesized renin.
AB - Rat renal cortical slices incubated in vitro released 7.0 ± 0.5% (mean ± SE, n = 30) of their total renin content into the incubation medium in 1 h. Isoproterenol (10-6 M), a β-adrenergic agonist, caused a 75 ± 17% (n = 7) increase in renin release from the cortical slices. Treatment of the cortical slices with either cycloheximide or puromycin caused a significant 96.2 ± 0.34 (n = 8) or 98.5 ± 0.3% (n = 5), respectively, inhibition of protein synthesis. After protein synthesis and presumably renin synthesis was blocked with either cycloheximide or puromycin, isoproterenol was still able to increase significantly renin release from the cortical slices despite almost total blockade of protein synthesis. We conclude that a storage pool of renin may exist, since 1) only 7.0 ± 0.5% of the cortical slice renin content is released each hour, and 2) isoproterenol stimulation of renin release is not acutely dependent on renin synthesis. It is hypothesized that β-adrenergic stimulation of renin release is elicited from a storage pool of previously synthesized renin.
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M3 - Article
C2 - 6753605
AN - SCOPUS:0020211768
SN - 0363-6143
VL - 12
SP - F434-F439
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
IS - 5
ER -