TY - JOUR
T1 - INDUCTION OF CHROMOSOME MOTION IN THE GLYCEROL‐ISOLATED MITOTIC APPARATUS
T2 - NUCLEOTIDE SPECIFICITY AND EFFECTS OF ANTIDYNEIN AND MYOSIN SERA ON THE MOTION
AU - SAKAI, HIKOICHI
AU - MABUCHI, ISSEI
AU - SHIMODA, SHUMEI
AU - KURIYAMA, RYOKO
AU - OGAWA, KAZUO
AU - MOHRI, HIDEO
PY - 1976
Y1 - 1976
N2 - Chromosome motion in glycerol‐isolated mitotic apparatus (MA) of sea urchin and starfish eggs was investigated with respect to nucleotide specificity and the effects of antisera against tryptic fragment (Fragment A) of flagellar dynein and starfish egg myosin. The motion was highly specific for ATP. GTP, ITP, CTP, UTP, and ADP caused no displacement of the chromosomes towards the poles. The anti‐Fragment A serum completely inhibited chromosome motion in the MA of the sea urchin egg, while antiserum against starfish egg myosin as well as its γ‐globulin fraction did not inhibit the motion in the isolated MA of the starfish egg, suggesting that chromosome motion depends upon dynein‐microtubule but not upon myosin‐actin interaction. In addition, colchicine completely suppressed the chromosome motion in vitro.
AB - Chromosome motion in glycerol‐isolated mitotic apparatus (MA) of sea urchin and starfish eggs was investigated with respect to nucleotide specificity and the effects of antisera against tryptic fragment (Fragment A) of flagellar dynein and starfish egg myosin. The motion was highly specific for ATP. GTP, ITP, CTP, UTP, and ADP caused no displacement of the chromosomes towards the poles. The anti‐Fragment A serum completely inhibited chromosome motion in the MA of the sea urchin egg, while antiserum against starfish egg myosin as well as its γ‐globulin fraction did not inhibit the motion in the isolated MA of the starfish egg, suggesting that chromosome motion depends upon dynein‐microtubule but not upon myosin‐actin interaction. In addition, colchicine completely suppressed the chromosome motion in vitro.
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U2 - 10.1111/j.1440-169X.1976.00211.x
DO - 10.1111/j.1440-169X.1976.00211.x
M3 - Article
AN - SCOPUS:0017060647
SN - 0012-1592
VL - 18
SP - 211
EP - 219
JO - Development, Growth & Differentiation
JF - Development, Growth & Differentiation
IS - 3
ER -