Induction of quorum sensing in microdroplets by transporting small molecule through PDMS

Jung Uk Shim, Santoshkumar N. Patil, James T. Hodgkinson, Steven D. Bowden, David R. Spring, Martin Welch, Wilhelm T S Huck, Florian Hollfelder, Chris Abell

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

By exploiting the permeability of PDMS fabricated on the microfluidic system, it is possible to deliver hormone-like small molecules into stored microdroplets encapsulating cells, which are harbouring a plasmid to detect quorum sensing molecules. The ability to change the condition of microdroplets provides a tool to investigate responses of individual cells under controllable chemical environments at a given initial condition. This study will be helpful to understand the heterogeneity of cells to different regulators during their lifecycle by simply switching the reservoir conditions in predetermined manners.

Original languageEnglish (US)
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages656-658
Number of pages3
Volume1
StatePublished - Dec 1 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/3/1010/7/10

Keywords

  • Control of environment
  • Microdroplet
  • Permeability of PDMS
  • Quorum sensing

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