In situ alkaline transesterification is reported to be an efficient method of biodiesel (methyl ester) production from several oilseed crops. Seed moisture content has a significant influence on methyl ester yields from biodiesel made in situ. Biodiesel quality properties are closely associated with the parent feed stock, and methyl esters derived from conventional transesterified canola oil have improved cold flow properties when compared to other oilseed crops. This study evaluated canola in situ alkaline transesterification and examined the impact of seed moisture content on methyl ester yield. Biodiesel quality parameters including oxidative stability, moisture content, kinematic viscosity, free fatty acids and total glycerin were evaluated between biodiesel derived from conventional and in situ methods.