TY - JOUR
T1 - Inhibition of β-glucuronidase by casein hydrolysate formula
AU - Gourley, Glenn R.
AU - Kreamer, Bill L.
AU - Cohnen, Monika
PY - 1997/9
Y1 - 1997/9
N2 - Background: A casein hydrolysate infant formula has been shown to be associated with lower levels of neonatal jaundice than are standard infant formulas. Because β-glucuronidase is related to neonatal jaundice, this study examined the effect of a casein hydrolysate formula on β- glucuronidase. Methods: β-glucuronidase activity was measured with or without added dietary components. The β-glucuronidase sources used were meconium, breast milk, and the purified bovine liver enzyme. The dietary components assayed for their effect on β-glucuronidase activity included casein hydrolysate formula (Nutramigen), whey-predominant formula (Enfamil), breast milk, enzymatically hydrolyzed casein, and other constituents of the casein hydrolysate formula. Stool samples of 6-day-old infants, who were exclusively fed one of the two formulas or breast milk, were also assayed for inhibition of β-glucuronidase. Results: Only Nutramigen, enzymatically hydrolyzed casein, and stool from Nutramigen-fed infants consistently demonstrated significant inhibition of β-glucuronidase activity, ranging from 45% to 85% of that in controls. The inhibition of β-glucuronidase in purified bovine liver demonstrates a dose response in a pH range from 4 to 7.3. Conclusions: Hydrolyzed casein contains a β-glucuronidase inhibitor that, in casein hydrolysate-fed infants, persists after passage through the digestive tract. These data are consistent with the possibility that inhibition of β-glucuronidase is a mechanism by which infants fed casein hydrolysate have lower jaundice levels than infants fed routine formulas or breast milk. Further study of this mechanism is needed.
AB - Background: A casein hydrolysate infant formula has been shown to be associated with lower levels of neonatal jaundice than are standard infant formulas. Because β-glucuronidase is related to neonatal jaundice, this study examined the effect of a casein hydrolysate formula on β- glucuronidase. Methods: β-glucuronidase activity was measured with or without added dietary components. The β-glucuronidase sources used were meconium, breast milk, and the purified bovine liver enzyme. The dietary components assayed for their effect on β-glucuronidase activity included casein hydrolysate formula (Nutramigen), whey-predominant formula (Enfamil), breast milk, enzymatically hydrolyzed casein, and other constituents of the casein hydrolysate formula. Stool samples of 6-day-old infants, who were exclusively fed one of the two formulas or breast milk, were also assayed for inhibition of β-glucuronidase. Results: Only Nutramigen, enzymatically hydrolyzed casein, and stool from Nutramigen-fed infants consistently demonstrated significant inhibition of β-glucuronidase activity, ranging from 45% to 85% of that in controls. The inhibition of β-glucuronidase in purified bovine liver demonstrates a dose response in a pH range from 4 to 7.3. Conclusions: Hydrolyzed casein contains a β-glucuronidase inhibitor that, in casein hydrolysate-fed infants, persists after passage through the digestive tract. These data are consistent with the possibility that inhibition of β-glucuronidase is a mechanism by which infants fed casein hydrolysate have lower jaundice levels than infants fed routine formulas or breast milk. Further study of this mechanism is needed.
KW - Bilirubin metabolism
KW - Infant nutrition
KW - Neonatal jaundice
KW - β-glucuronidase inhibition
UR - http://www.scopus.com/inward/record.url?scp=0030846743&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030846743&partnerID=8YFLogxK
U2 - 10.1097/00005176-199709000-00005
DO - 10.1097/00005176-199709000-00005
M3 - Article
C2 - 9285376
AN - SCOPUS:0030846743
SN - 0277-2116
VL - 25
SP - 267
EP - 272
JO - Journal of pediatric gastroenterology and nutrition
JF - Journal of pediatric gastroenterology and nutrition
IS - 3
ER -