Inhibition of Autographa californica nucleopolyhedrovirus (AcNPV) polyhedrin gene expression by DNAzyme knockout of its serine/threonine kinase (pk1) gene

Gourav Mishra, Pooja Chadha, Indrajit Chaudhury, Rakha H. Das

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

DNAzyme is known to selectively cleave RNA at predetermined site. Transfection of Autographa californica nucleopolyhedrovirus (AcNPV) infected Sf9 cells with serine/threonine kinase (pk1) mRNA specific DNAzymes, DZ1 and DZ2 to cleave the viral coded (pk1) mRNA in between 87th and 88th, and 250th and 251st nucleotide, respectively inhibited the pk1 mRNA and its protein expressions. Interestingly, polh mRNA and protein expressions were also inhibited by these DNAzymes despite their inability to cleave polh mRNA. The polyhedrin promoter driven green fluorescent protein (GFP) mRNA and protein expressions were also inhibited by these pk1 specific DZs. Surprisingly the extents of inhibition of polyhedrin and GFP at different concentrations of both DZs were higher than that of pk1 mRNA and protein expressions. These results suggested that pk1 regulates polyhedrin promoter driven transcription of AcNPV, and the effect of one gene expression on that of other can be studied by DNAzyme knockdown.

Original languageEnglish (US)
Pages (from-to)197-201
Number of pages5
JournalVirus research
Volume135
Issue number1
DOIs
StatePublished - Jul 2008

Bibliographical note

Funding Information:
Financial support from the Council of Scientific and Industrial Research (CSIR), India and Infrastructural facilities provided by Director IGIB to carry out this research work are gratefully acknowledged. I.C. acknowledges the financial support from the University Grants Commission, India.

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

  • Autographa californica nucleopolyhedrovirus
  • DNAzyme
  • Polyhedrin gene
  • Serine/threonine kinase

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