TY - JOUR
T1 - Inhibition of pulmonary surfactant adsorption by serum and the mechanisms of reversal by hydrophilic polymers
T2 - Theory
AU - Zasadzinski, Joseph A.
AU - Alig, T. F.
AU - Alonso, Coralie
AU - De La Serna, Jorge Bernardino
AU - Perez-Gil, Jesus
AU - Taeusch, H. William
PY - 2005/9
Y1 - 2005/9
N2 - A theory based on the Smolukowski analysis of colloid stability shows that the presence of charged, surface-active serum proteins at the alveolar air-liquid interface can severely reduce or eliminate the adsorption of lung surfactant from the subphase to the interface, consistent with the observations reported in the companion article (pages 1769-1779). Adding nonadsorbing, hydrophilic polymers to the subphase provides a depletion attraction between the surfactant aggregates and the interface, which can overcome the steric and electrostatic resistance to adsorption induced by serum. The depletion force increases with polymer concentration as well as with polymer molecular weight. Increasing the surfactant concentration has a much smaller effect than adding polymer, as is observed. Natural hydrophilic polymers, like the SP-A present in native surfactant, or hyaluronan, normally present in the alveolar fluids, can enhance adsorption in the presence of serum to eliminate inactivation.
AB - A theory based on the Smolukowski analysis of colloid stability shows that the presence of charged, surface-active serum proteins at the alveolar air-liquid interface can severely reduce or eliminate the adsorption of lung surfactant from the subphase to the interface, consistent with the observations reported in the companion article (pages 1769-1779). Adding nonadsorbing, hydrophilic polymers to the subphase provides a depletion attraction between the surfactant aggregates and the interface, which can overcome the steric and electrostatic resistance to adsorption induced by serum. The depletion force increases with polymer concentration as well as with polymer molecular weight. Increasing the surfactant concentration has a much smaller effect than adding polymer, as is observed. Natural hydrophilic polymers, like the SP-A present in native surfactant, or hyaluronan, normally present in the alveolar fluids, can enhance adsorption in the presence of serum to eliminate inactivation.
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U2 - 10.1529/biophysj.105.062646
DO - 10.1529/biophysj.105.062646
M3 - Article
C2 - 16006630
AN - SCOPUS:24144458184
SN - 0006-3495
VL - 89
SP - 1621
EP - 1629
JO - Biophysical journal
JF - Biophysical journal
IS - 3
ER -