Abstract
A cell line stably transfected with reporter genes activated by retinoic acid was used to test a paper mill effluent for the presence of retinoids or components that interfere with retinoic acid-stimulated gene transcription. No retinoids were detected in effluent or control water. However, effluent water significantly decreased reporter activity stimulated by all-trans-retinoic acid, while activity stimulated by 9-cis-retinoic acid was unaffected. In a limited fractionation through a C-18 solid phase-exchange column the inhibitory activity was retained in the aqueous fraction, indicating that the activity was polar.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-4 |
| Number of pages | 4 |
| Journal | Environmental Pollution |
| Volume | 119 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 20 2002 |
Keywords
- RAR
- RXR
- Transfected cells