Inner ear damage and passage through the round window membrane of Pseudomonas Aeruginosa exotoxin a in a chinchilla model

Lars Lundman, Teruhiko Harada, Peter A Santi, Steven K. Juhn, Tetsuo Morizono, Dan Bagger-Sjöbäck

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

By the use of computer-assisted morphometric analysis of the organ of Corti and/or measurements of action potential threshold changes, inner ear changes in chinchillas were evaluated 4 weeks after application on the round window membrane of a Pseudomonas aeruginosa exotoxin A solution. Severe inner ear damage was detected after application of 50 ng (5 μL at a concentration of 10 μg/mL) exotoxin A, whereas application of 5 ng exotoxin did not cause measurable inner ear damage. Perilymph concentrations of exotoxin A were measured with an enzyme-linked immunosorbent assay 1.5 to 19 hours after 50 ng, 0.5 μg, or 5 μg of exotoxin A was applied on the round window membrane. Only the highest concentration produced measurable levels of exotoxin in the inner ear fluids. It is concluded that exotoxin A present on the round window membrane of the chinchilla has the ability to penetrate into the inner ear and cause irreversible inner ear changes.

Original languageEnglish (US)
Pages (from-to)437-444
Number of pages8
JournalAnnals of Otology, Rhinology & Laryngology
Volume101
Issue number5
DOIs
StatePublished - May 1992

Bibliographical note

Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.

Keywords

  • Pseudomonas aeruginosa exotoxin A
  • round window membrane permeability
  • sensorineural hearing loss

Fingerprint Dive into the research topics of 'Inner ear damage and passage through the round window membrane of Pseudomonas Aeruginosa exotoxin a in a chinchilla model'. Together they form a unique fingerprint.

Cite this