Insulin-Degrading Enzyme in a Human Colon Adenocarcinoma Cell Line (Caco-2)

Jane P.F. Bai, Matt J.P. Hsu, W. Thomas Shier

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The activity of insulin-degrading enzyme (IDE), a thiol metalloprotease degrading insulin in many insulin target cells, was determined in human colon adenocarcinoma (Caco-2) cells. Insulin-degrading activity was localized in the cytosol of Caco-2 cells, accounting for 88% of total activity. Western blots and immunoprecipitation showed that IDE was present in the cytosol of Caco-2 cells and contributed to more than 93% cytosolic insulin-degrading activity. Cytosolic insulin degradation was strongly inhibited by IDE inhibitors, including N-ethylmaleimide, 1,10-phenanthroline, p-chloromericuribenzoate, and EDTA, but was not significantly or not as extensively inhibited by strong inhibitors of proteasome, i.e., chymostatin, soybean trypsin inhibitor, leupeptin, and Dip-F. These results suggest that IDE is present in Caco-2 cells, that Caco-2 IDE has properties similar to those of its counterparts in insulin-target tissues, and that it significantly contributes to intracellular insulin degradation.

Original languageEnglish (US)
Pages (from-to)513-517
Number of pages5
JournalPharmaceutical Research: An Official Journal of the American Association of Pharmaceutical Scientists
Volume12
Issue number4
DOIs
StatePublished - Apr 1995

Keywords

  • Caco-2 cells
  • insulin-degrading enzyme

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