1. 1. Diabetes had no significant effect on IGFBP-3 message and serum levels however, subsequent insulin treatment caused more than a two-fold increase in both hepatic IGFBP-3 mRNA and serum levels above controls (P < 0.05). 2. 2. The induction of diabetes in pigs significantly increased the steady state levels of IGFBP-2 mRNA in the liver of young swine (P < 0.05). 3. 3. Both liver message and serum IGFBP-2 were reduced to control levels with insulin therapy. 4. 4. We report here that in addition to its affects on IGFBP-2, insulin is involved in the regulation of IGFBP-3 expression.
|Original language||English (US)|
|Number of pages||7|
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|State||Published - Oct 1993|
Bibliographical noteFunding Information:
In biological fluids, IGFs are complexed to specific binding proteins which can modulate their biological action (Elgin et al., 1987; Clemmons et al., 1987; De Vroede et al., 1986; De Mellow and Baxter, 1988; Cornell et al., 1987; Gopinath et al., 1989; Walton et aL, 1989; Blum et al., 1989). Postnatally, the majority of the circulating IGF is bound to IGFBP-3 in the 150 kDa IGF binding complex (Baxter, 1991). Presently the cDNAs for six different IGFBPs (IGFBP-1 through 6) have been cloned and sequenced (Shimasaki et al., 1991a,b). Two of the predominant IGFBPs found in porcine serum are IGFBP-3 and -2 (Coleman et al., 1991). Circulating levels of these two IGFBPs have been shown to be differentially regulated by growth hormone and nutri- *Published as article No. 20,061 of the Scientific Journal Series of the Minnesota Agricultural Experiment station, Project No. 16-068. Supported in part by USDA Grant No. 87-37265-2598. tTo whom correspondence should be addressed at: Depart-ment of Animal Science, University of Minnesota, 137 Peters Hall, 1404 Gortner Ave., St. Paul, MN 55108, U.S.A.
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