Insulin Receptor Substrate Suppression by the Tyrphostin NT157 Inhibits Responses to Insulin-Like Growth Factor-I and Insulin in Breast Cancer Cells

Yuzhe Yang, Jie Ying Chan, Nuri A. Temiz, Douglas Yee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Insulin and insulin-like growth factor (IGF) signaling systems regulate breast cancer growth, progression, and metastasis. The insulin receptor substrates 1 and 2 (IRS1/2) transduce signaling from the type I IGF receptor (IGF-IR) and insulin receptor (InR) to mediate the biological effects of receptor activation. In breast cancer, IRS-1 plays a critical role in cancer cell proliferation while IRS-2 is associated with motility and metastasis. NT157, a small-molecule tyrphostin, downregulates IRS proteins in several model systems. In breast cancer cells, NT157 treatment suppressed IRS protein expression in a dose-dependent manner. Exposure to NT157 inhibited the activation of downstream signaling mediated by the IRS proteins. NT157 induced a MAPK-dependent serine phosphorylation of IRS proteins which resulted in disassociation between IRS proteins and their receptors resulting in IRS degradation. In estrogen receptor-α-positive (ERα+) breast cancer cells (MCF-7 and T47D), NT157 also resulted in cytoplasmic ERα downregulation likely because of disruption of an IRS-1-IGF-IR/InR/ERα complex. NT157 decreased S phase fraction, monolayer, and anchorage-independent growth after IGF/insulin treatment in ERα+ breast cancer cells. NT157 downregulation of IRS protein expression also sensitized ERα+ breast cancer cells to rapamycin. Moreover, NT157 inhibited the growth of tamoxifen-resistant ERα+ breast cancer cells. Given that both IGF-IR and InR play a role in cancer biology, targeting of IRS adaptor proteins may be a more effective strategy to inhibit the function of these receptors.

Original languageEnglish (US)
Pages (from-to)371-382
Number of pages12
JournalHormones and Cancer
Volume9
Issue number6
DOIs
StatePublished - Dec 1 2018

Bibliographical note

Funding Information:
Funding Information This study was supported by Komen for the Cure SAC110039 (DY), Mayo Clinic SPORE in Breast Cancer (NIH/NCI P50CA116201-06A1 - Ingle), and NIH/NCI Cancer Center Support Grant (2P30-CA077598 - Yee).

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.

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