Interaction between two essential, conserved bacterial proteins YeaZ and glycoprotease as a potential antibacterial target in multi-drug-resistant Staphylococcus aureus

Timmie A. Britton, Haiyong Guo, Yinduo Ji

Research output: Contribution to journalReview articlepeer-review

Abstract

Protein–protein interactions among highly conserved and essential proteins can serve as new targets for antibacterial therapies. One protein–protein interaction between two widely conserved and essential bacterial proteins, YeaZ and its paralog, a putative glycoprotease, is being looked into for its antimicrobial drug potential. These two proteins possess tandem functions, including repression of the branched-chain amino acids biosynthesis and induction of a tRNA modification important in enhancing translation fidelity through anticodon–codon base pairing. Heterodimer formation between these two proteins is essential for Staphylococcus aureus, and other bacterial species including Escherichia coli and Salmonella typhimurium. Such YeaZ–glycoprotease interaction could thus be a target for antimicrobial drugs designed for multi-drug-resistant S. aureus. In this review, we discuss the function, structure, and interaction between these two proteins and their orthologs in other bacteria.

Original languageEnglish (US)
JournalScience Progress
Volume103
Issue number1
DOIs
StatePublished - Jan 2020

Bibliographical note

Funding Information:
Staphylococcus aureus Britton Timmie A 1 Guo Haiyong 2 https://orcid.org/0000-0002-0199-0195 Ji Yinduo 3 1 College of Biomedical Science, University of Minnesota, Minneapolis, MN, USA 2 College of Life Science, Jilin Normal University, Siping, China 3 Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Minneapolis, MN, USA Yinduo Ji, Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Ave., St Paul, Minneapolis, MN 55108, USA. Email: jixxx002@umn.edu 11 2019 0036850419890521 © The Author(s) 2019 2019 SAGE Publications This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License ( http://www.creativecommons.org/licenses/by-nc/4.0/ ) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages ( https://us.sagepub.com/en-us/nam/open-access-at-sage ). Protein–protein interactions among highly conserved and essential proteins can serve as new targets for antibacterial therapies. One protein–protein interaction between two widely conserved and essential bacterial proteins, YeaZ and its paralog, a putative glycoprotease, is being looked into for its antimicrobial drug potential. These two proteins possess tandem functions, including repression of the branched-chain amino acids biosynthesis and induction of a tRNA modification important in enhancing translation fidelity through anticodon–codon base pairing. Heterodimer formation between these two proteins is essential for Staphylococcus aureus , and other bacterial species including Escherichia coli and Salmonella typhimurium . Such YeaZ–glycoprotease interaction could thus be a target for antimicrobial drugs designed for multi-drug-resistant S. aureus . In this review, we discuss the function, structure, and interaction between these two proteins and their orthologs in other bacteria. Staphylococcus aureus antibacterial target YeaZ glycoprotease protein–protein interaction General Agricultural Research fund for EZID Signature Program project MIN-63-075 National Natural Science Foundation of China https://doi.org/10.13039/501100001809 31772768 edited-state corrected-proof The authors would like to thank Dr Laurie Parke for her careful review of the manuscript and constructive suggestions. Declaration of conflicting interests The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. Funding The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was partially supported by the award of MIN-63-075 (to Y.J.) from the General Agricultural Research fund for EZID Signature Program project in the College of Veterinary Medicine at the University of Minnesota and by the grant no. 31772768 (to Y.J.) from the National Natural Science Foundation of China. ORCID iD Yinduo Ji https://orcid.org/0000-0002-0199-0195

Funding Information:
The authors would like to thank Dr Laurie Parke for her careful review of the manuscript and constructive suggestions. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was partially supported by the award of MIN-63-075 (to Y.J.) from the General Agricultural Research fund for EZID Signature Program project in the College of Veterinary Medicine at the University of Minnesota and by the grant no. 31772768 (to Y.J.) from the National Natural Science Foundation of China.

Publisher Copyright:
© The Author(s) 2020.

Keywords

  • Staphylococcus aureus
  • YeaZ
  • antibacterial target
  • glycoprotease
  • protein–protein interaction

PubMed: MeSH publication types

  • Journal Article

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