Abstract
The effect of 4-aminopyridine and its analogs on the specific binding of [3H]phencyclidine was investigated in rat brain homogenates. 4-Aminopyridine (4-AP) and 3,4-diaminopyridine displaced [3H]phencyclidine binding, while 3-aminopyridine was without effect. The concentrations of 4-AP required for inhibition of binding increased with increasing the ligand concentration, and the resultant Dixon plots indicated a competitive type of interaction. However, 4-AP also accelerated the dissociation rate of the ligand-receptor complex, suggesting that the effect of 4-AP on phencyclidine receptors in the brain might not be purely competitive.
Original language | English (US) |
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Pages (from-to) | 87-91 |
Number of pages | 5 |
Journal | Neuroscience Letters |
Volume | 67 |
Issue number | 1 |
DOIs | |
State | Published - Jun 6 1986 |
Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by BRS Grant S07-RR05770. The authors would like to thank Ms. Renee Powe and Mrs. Pat Tretter for their excellent secretarial help, and Mr. Michael Gentry for his assistance.
Keywords
- 4-aminopyridine
- phencyclidine (PCP)
- potassium channel
- rat