TY - JOUR
T1 - Intercellular junctions and other cell surface differentiations of H4-IIE hepatoma cells in vitro
AU - Porvaznik, Martin
AU - Johnson, Ross G.
AU - Sheridan, Judson D.
N1 - Funding Information:
This work was supported by grants from the National Cancer Institute RO1-CA 11114 and PO1-CA 16228.
PY - 1976/6
Y1 - 1976/6
N2 - Cells of the H4-II-E line retain a number of the differentiated features of normal hepatocytes. Individual cultures contain both monolayer and stratified regions. Utilizing a variety of electron microscopic (EM) techniques, intercellular junctions and surface differentiations in both regions of the cultures were investigated. Gap junctions resembled those in normal liver, but were smaller and occurred infrequently. In situ fracturing methods revealed rectilinear arrays of particles in the apical (exposed) surfaces of the cultures. The tight junction forms varied in complexity ranging from linear fibrils to continuous, circular forms that appeared to occlude extracellular, spherical pockets between individual pairs of cells. The complex forms, particularly the continuous, circular forms, were more commonly found in stratified regions of the culture. Microvilli extended into the pockets, which probably correspond to structures seen in sections. The pockets excluded colloidal lanthanum and their membranes were strongly stained with the Wachstein-Meisel ATPase method.
AB - Cells of the H4-II-E line retain a number of the differentiated features of normal hepatocytes. Individual cultures contain both monolayer and stratified regions. Utilizing a variety of electron microscopic (EM) techniques, intercellular junctions and surface differentiations in both regions of the cultures were investigated. Gap junctions resembled those in normal liver, but were smaller and occurred infrequently. In situ fracturing methods revealed rectilinear arrays of particles in the apical (exposed) surfaces of the cultures. The tight junction forms varied in complexity ranging from linear fibrils to continuous, circular forms that appeared to occlude extracellular, spherical pockets between individual pairs of cells. The complex forms, particularly the continuous, circular forms, were more commonly found in stratified regions of the culture. Microvilli extended into the pockets, which probably correspond to structures seen in sections. The pockets excluded colloidal lanthanum and their membranes were strongly stained with the Wachstein-Meisel ATPase method.
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U2 - 10.1016/S0022-5320(76)80092-5
DO - 10.1016/S0022-5320(76)80092-5
M3 - Article
C2 - 132534
AN - SCOPUS:0017194937
SN - 0022-5320
VL - 55
SP - 343
EP - 359
JO - Journal of Ultrasructure Research
JF - Journal of Ultrasructure Research
IS - 3
ER -