Interconversion between three overstretched DNA structures

Xinghua Zhang, Yuanyuan Qu, Hu Chen, Ioulia Rouzina, Shengli Zhang, Patrick S. Doyle, Jie Yan

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Double-stranded DNA can exist in multiple structures, including three recently identified overstretched structures (peeled ssDNA, DNA bubble, and S-DNA) for torsion-unconstrained DNA under large tension. Here, we report systematic studies of interconversion between these overstretched DNA structures induced by changing NaCl concentration at constant force. At forces above 70 pN where DNA exists in one of the overstretched states, direct interconversions between S-DNA and DNA bubble for the end-closed DNA construct, as well as interconversions between S-DNA and peeled ssDNA for the end-opened DNA construct, were observed to involve stepwise extension changes. Interestingly, compared to other conversions, the conversion from peeled ssDNA to S-DNA has ultraslow kinetics, which can be explained by formation of secondary hairpin structures on a relaxed strand of peeled ssDNA. Our findings provide important insights into the structures of torsion-unconstrained DNA under large force.

Original languageEnglish (US)
Pages (from-to)16073-16080
Number of pages8
JournalJournal of the American Chemical Society
Volume136
Issue number45
DOIs
StatePublished - Nov 12 2014

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