Interfacial interactions of ceramide with dimyristoylphosphatidylcholine: Impact of the N-acyl chain

Juha M. Holopainen, Howard L. Brockman, Rhoderick E Brown, Paavo K.J. Kinnunen

Research output: Contribution to journalArticlepeer-review

91 Scopus citations

Abstract

The mixing behavior of dimyristoylphosphatidylcholine (DMPC) with either N-palmitoyl-sphingosine (C16:0-ceramide) or N-nervonoyl-sphingosine (C24:1-ceramide) was examined using monomolecular films. While DMPC forms highly elastic liquid-expanded monolayers, both neat C16:0-ceramide and C24:1-ceramide yield stable solid condensed monomolecular films with small areas and low interracial elasticity. Compression isotherms of mixed C16:0-ceramide/DMPC films exhibit an apparent condensation upon increasing Xcer16:0 at all surface pressures. The average area isobars, coupled with the lack of a liquid-expanded to condensed phase transition as Xcer16:0 is increased, are indicative of immiscibility of the lipids at all surface pressures. In contrast, isobars for C24:1-ceramide/DMPC mixtures show surface pressure-dependent apparent condensation or expansion and surface pressure-area isotherms show a composition and surface pressure-dependent phase transition. This suggests miscibility, albeit non-ideal, of C24:1-ceramide and DMPC in both liquid and condensed surface phases. The above could be verified by fluorescence microscopy of the monolayers and measurements of surface potential, which revealed distinctly different domain morphologies and surface potential values for the DMPC/C16:0- and DMPC/C24:1-ceramide monolayers. Taken together, whereas C16:0-ceramide and DMPC form immiscible pseudo-compounds, C24:1-ceramide and DMPC are partially miscible in both the liquid-expanded and condensed phases, and a composition and lateral pressure-dependent two-phase region is evident between the liquid-expanded and condensed regimes. Our results provide novel understanding of the regulation of membrane properties by ceramides and raise the possibility that ceramides with different acyl groups could serve very different functions in cells, relating to their different physicochemical properties.

Original languageEnglish (US)
Pages (from-to)765-775
Number of pages11
JournalBiophysical journal
Volume80
Issue number2
DOIs
StatePublished - 2001

Bibliographical note

Funding Information:
This study was supported by Finnish State Medical Research Council and TEKES (P.K.J.K.), and USPHS Grants HL49180 (H.L.B.) and GM45928 (R.E.B.). J.M.H. is supported by the M.D./Ph.D. program of University of Helsinki.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Fingerprint

Dive into the research topics of 'Interfacial interactions of ceramide with dimyristoylphosphatidylcholine: Impact of the N-acyl chain'. Together they form a unique fingerprint.

Cite this