Abstract
Formalin-inactivated respiratory syncytial virus (FI-RSV) vaccination has been associated with severe disease in humans. Research in mice suggests that FI-RSV may prime for decreased interferon gamma (IFN-γ) production at subsequent infection. Interferon-γ production by peripheral blood mononuclear cells (PBMC) was measured following challenge of calves vaccinated with FI-BRSV to determine whether a similar mechanism is operative in a host naturally susceptible to RSV. Eight-week old male Holstein calves were administered FI-BRSV and mock challenge (V/M, n = 6); mock vaccination and BRSV challenge (M/C, n = 6) or FI-BRSV and BRSV challenge (V/C, n = 7). Vaccine was administered twice at a 2-week interval; challenge followed one month later. On days 0, 5 and 10 postchallenge (PC), PBMC were stimulated in vitro for 24 h with live BRSV, concanavalin A (positive control) or spent media (negative control). Supernatants were assayed for IFN-γ using ELISA. Interferon-γ production by BRSV-stimulated PBMC was increased in M/C and V/C calves as compared to V/M calves on day 5 PC (p < 0.015); and increased in M/C calves compared to V/C and V/M Calves on day 10 PC (p < 0.015). Over time postchallenge, a significant increase in IFN-γ production by BRSV-stimulated PBMC was seen in M/C calves (p < 0.025) but not in V/C calves. FI-BRSV vaccination of calves led to diminished IFN-γ production postchallenge. Decreased IFN-γ production may have contributed to impaired viral clearance and enhanced disease in FI-BRSV vaccinated calves.
Original language | English (US) |
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Pages (from-to) | 1293-1297 |
Number of pages | 5 |
Journal | Vaccine |
Volume | 17 |
Issue number | 11-12 |
DOIs | |
State | Published - Mar 1999 |
Bibliographical note
Funding Information:This work was supported by a grant from National Institutes of Health (ROI AI3 7213-01) from the Institute of Allergy and Infections Diseases.
Keywords
- BRSV
- Formalin-inactivated RSV
- Interferon gamma