Abstract
PROBLEM: Intrauterine infection accounts for 20% of preterm labor and results in the production of decidual inflammatory cytokines, including interleukin-1 (IL-1). The oxytocin receptor plays a key role in the onset of preterm labor. Cytokines likely regulate oxytocin receptor expression through several cytokine-induced DNA-binding proteins. METHOD OF STUDY: The objective of this study was to evaluate the effect of the IL-1 alone on oxytocin receptor number as measured by radioligand binding and immunocytochemistry, and oxytocin receptor mRNA as measured by reverse transriptase-polymerase chain reaction (RT-PCR) in cultured uterine myocytes. RESULTS: Unexpectedly, IL-1 treatment decreased oxytocin receptor number from 111,067 to 23,941 receptors/cell. Loss of oxytocin receptor binding began after 8 hr of IL-1 treatment and was reversible after IL-1 removal. Immunocytochemistry confirmed a loss of cellular oxytocin receptors. Oxytocin receptor mRNA decreased beginning after 2 hr of IL-1 treatment. CONCLUSIONS: IL-1 down-regulates the uterine oxytocin receptor in a time- and dose-dependent fashion.
Original language | English (US) |
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Pages (from-to) | 85-91 |
Number of pages | 7 |
Journal | American Journal of Reproductive Immunology |
Volume | 43 |
Issue number | 2 |
State | Published - Feb 2000 |
Keywords
- Human uterus
- Interleukin-1β
- Oxytocin receptor