Intersectin is a negative regulator of dynamin recruitment to the synaptic endocytic zone in the central synapse

Emma Evergren, Helge Gad, Kristin Walther, Anna Sundborger, Nikolay Tomilin, Oleg Shupliakov

Research output: Contribution to journalArticlepeer-review

66 Scopus citations


Intersectin is a multidomain dynamin-binding protein implicated in numerous functions in the nervous system, including synapse formation and endocytosis. Here,wedemonstrate that during neurotransmitter release in the central synapse, intersectin, like its binding partner dynamin, is redistributed from the synaptic vesicle pool to the periactive zone. Acute perturbation of the intersectin-dynamin interaction by microinjection of either intersectin antibodies or Src homology 3 (SH3) domains inhibited endocytosis at the fission step. Although the morphological effects induced by the different reagents were similar, antibody injections resulted in a dramatic increase in dynamin immunoreactivity around coated pits and at constricted necks, whereas synapses microinjected with the GST (glutathione S-transferase)-SH3C domain displayed reduced amounts of dynamin in the neck region. Our data suggest that intersectin controls the amount of dynamin released from the synaptic vesicle cluster to the periactive zone and that it may regulate fission of clathrin-coated intermediates.

Original languageEnglish (US)
Pages (from-to)379-390
Number of pages12
JournalJournal of Neuroscience
Issue number2
StatePublished - Jan 10 2007
Externally publishedYes


  • Glutamatergic neurons
  • Periactive zone
  • Presynaptic mechanisms
  • Spinal cord
  • Synaptic vesicle recycling
  • Ultrastructure


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