TY - JOUR
T1 - Introducing Students to Protein Analysis Techniques
T2 - Separation and Comparative Analysis of Gluten Proteins in Various Wheat Strains
AU - Pirinelli, Alyssa L.
AU - Trinidad, Jonathan C.
AU - Pohl, Nicola L.B.
PY - 2016/2/9
Y1 - 2016/2/9
N2 - Polyacrylamide gel electrophoresis (PAGE) is commonly taught in undergraduate laboratory classes as a traditional method to analyze proteins. An experiment has been developed to teach these basic protein gel skills in the context of gluten protein isolation from various types of wheat flour. A further goal is to relate this technique to current mass spectrometry-based proteomic analysis techniques. Gluten proteins serve as particularly good experimental systems for the undergraduate classroom given the low cost of their sources and the current widespread public interest in gluten-free diets and Celiac-Sprue disease. The experiment set also can serve as preliminary data for student-written original research proposals and be the basis for a discussion of how scientific topics are presented in public forums. This experiment can also be readily adapted to a range of audiences and facilities.
AB - Polyacrylamide gel electrophoresis (PAGE) is commonly taught in undergraduate laboratory classes as a traditional method to analyze proteins. An experiment has been developed to teach these basic protein gel skills in the context of gluten protein isolation from various types of wheat flour. A further goal is to relate this technique to current mass spectrometry-based proteomic analysis techniques. Gluten proteins serve as particularly good experimental systems for the undergraduate classroom given the low cost of their sources and the current widespread public interest in gluten-free diets and Celiac-Sprue disease. The experiment set also can serve as preliminary data for student-written original research proposals and be the basis for a discussion of how scientific topics are presented in public forums. This experiment can also be readily adapted to a range of audiences and facilities.
KW - Biochemistry
KW - Communication/Writing
KW - Electrophoresis
KW - Hands-On Learning/Manipulatives
KW - Inquiry-Based/Discovery Learning
KW - Laboratory Instruction
KW - Mass Spectrometry
KW - Proteins/Peptides
KW - Upper-Division Undergraduate
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U2 - 10.1021/acs.jchemed.5b00311
DO - 10.1021/acs.jchemed.5b00311
M3 - Article
AN - SCOPUS:84957968427
VL - 93
SP - 330
EP - 334
JO - Journal of Chemical Education
JF - Journal of Chemical Education
SN - 0021-9584
IS - 2
ER -