Abstract
Removal of calcium from cultures of bovine splenic lymphoid cells with ethyleneglycol-bis-(2-amino ethylether)-N,N'-tetraacetic acid (EGTA) caused significant suppression of pokeweed mitogen-induced immunoglobulin (Ig) biosynthesis. It was found that removal of calcium for 48 hours of a 5-day culture significantly altered Ig biosynthesis. Re-addition of calcium after 48 hours did not restore Ig synthesis. The EGTA caused significant (P < 0.05) suppression of DNA synthesis when calcium was removed from concanavalin A- and purified protein derivative-stimulated peripheral blood lymphocyte cultures. Further, removal of calcium for 48 hours of 4-day cultures significantly altered the DNA synthesis in mitogen- and antigen-stimulated cultures.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1319-1322 |
| Number of pages | 4 |
| Journal | American journal of veterinary research |
| Volume | 41 |
| Issue number | 8 |
| State | Published - Aug 1 1980 |