Ion mobility spectrometry was utilized to corroborate the identity of streptorubin B (2) as the natural product produced by Streptomyces coelicolor. Natural product 2 was initially assigned as butylcycloheptylprodigiosin (3), and only relatively recently was this assignment clarified. We present additional evidence of this assignment by comparing collisional cross sections (ω) of synthetic standards of 2, 3, and metacycloprodigiosin (4) to the cyclic prodiginine produced by S. coelicolor. Calculated theoretical ω values demonstrate that cyclic prodiginines could be identified without standards. This work highlights ion mobility as an efficient tool for the dereplication of natural products.
Bibliographical noteFunding Information:
This work was supported by an NSF CAREER Award (to E.E.C.), a Sloan Research Fellow Award (to E.E.C.), the National Institutes of Health (R01GM085322 and R01GM124089 to R.J.T.), and the University of Minnesota.
Mass spectrometry analysis was performed, in part, at The University of Minnesota Department of Chemistry Mass Spectrometry Laboratory (MSL), supported by the Office of the Vice President of Research, College of Science and Engineering, and the Department of Chemistry at the University of Minnesota. The content of this paper is the sole responsibility of the authors and does not represent endorsement by the MSL.
© 2020 American Chemical Society and American Society of Pharmacognosy.
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PubMed: MeSH publication types
- Journal Article
- Research Support, N.I.H., Extramural
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, Non-P.H.S.