TY - JOUR
T1 - Isolation and characterization of a Rickettsia from the ovary of a Western black-legged tick, Ixodes pacificus
AU - Alowaysi, Maryam
AU - Chen, Junyan
AU - Stark, Sierra
AU - Teague, Kristine
AU - LaCourse, Monique
AU - Proctor, Joanna
AU - Vigil, Katie
AU - Corrigan, Jeremy
AU - Harding, Aja
AU - Li, Jinze
AU - Kurtti, Timothy
AU - Zhong, Jianmin
N1 - Publisher Copyright:
© 2019 Elsevier GmbH
PY - 2019/6
Y1 - 2019/6
N2 - A rickettsial isolate was obtained from a partially engorged Ixodes pacificus female, which was collected from Humboldt County, California. The isolate was provisionally named Rickettsia endosymbiont Ixodes pacificus (REIP). The REIP isolate displayed the highest nucleotide sequence identity to Rickettsia species phylotype G021 in I. pacificus (99%, 99%, and 100% for ompA, 16S rRNA, and gltA, respectively), a bacterium that was previously identified in I. pacifiucs by PCR. Analysis of sequences from complete opening frames of five genes, 16S rRNA, gltA, ompA, ompB, and sca4, provided inference to the bacteria's classification among other Rickettsia species. The REIP isolate displayed 99.8%, 99.4%, 99.2%, 99.5%, and 99.6% nucleotide sequence identity for 16S rRNA, gltA, ompA, ompB, and sca4 gene, respectively, with genes of ‘R. monacensis’ str. IrR/Munich, indicating the REIP isolate is closely related to ‘R. monacensis’. Our suggestion was further supported by phylogenetic analysis using concatenated sequences of 16S rRNA, gltA, ompA, ompB, and sca4 genes, concatenated sequences of dksA-xerC, mppA-purC, and rpmE-tRNAfMet intergenic spacer regions. Both phylogenetic trees implied that the REIP isolate is most closely related to ‘R. monacensis’ str. IrR/Munich. We propose the bacterium be considered as ‘Rickettsia monacensis’ str. Humboldt for its closest phylogenetic relative (=DSM 103975 T = ATCC TSD-94 T).
AB - A rickettsial isolate was obtained from a partially engorged Ixodes pacificus female, which was collected from Humboldt County, California. The isolate was provisionally named Rickettsia endosymbiont Ixodes pacificus (REIP). The REIP isolate displayed the highest nucleotide sequence identity to Rickettsia species phylotype G021 in I. pacificus (99%, 99%, and 100% for ompA, 16S rRNA, and gltA, respectively), a bacterium that was previously identified in I. pacifiucs by PCR. Analysis of sequences from complete opening frames of five genes, 16S rRNA, gltA, ompA, ompB, and sca4, provided inference to the bacteria's classification among other Rickettsia species. The REIP isolate displayed 99.8%, 99.4%, 99.2%, 99.5%, and 99.6% nucleotide sequence identity for 16S rRNA, gltA, ompA, ompB, and sca4 gene, respectively, with genes of ‘R. monacensis’ str. IrR/Munich, indicating the REIP isolate is closely related to ‘R. monacensis’. Our suggestion was further supported by phylogenetic analysis using concatenated sequences of 16S rRNA, gltA, ompA, ompB, and sca4 genes, concatenated sequences of dksA-xerC, mppA-purC, and rpmE-tRNAfMet intergenic spacer regions. Both phylogenetic trees implied that the REIP isolate is most closely related to ‘R. monacensis’ str. IrR/Munich. We propose the bacterium be considered as ‘Rickettsia monacensis’ str. Humboldt for its closest phylogenetic relative (=DSM 103975 T = ATCC TSD-94 T).
KW - Ixodes pacificus
KW - Multi-spacer sequence typing
KW - Multilocus sequence typing
KW - Phylogenetic analysis
KW - ‘Rickettsia monacensis’ str. Humboldt
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U2 - 10.1016/j.ttbdis.2019.04.017
DO - 10.1016/j.ttbdis.2019.04.017
M3 - Article
C2 - 31056486
AN - SCOPUS:85064897968
SN - 1877-959X
VL - 10
SP - 918
EP - 923
JO - Ticks and Tick-borne Diseases
JF - Ticks and Tick-borne Diseases
IS - 4
ER -