Following treatment of cultured mosquito cells (Aedes albopictus line of Singh) with the chemical mutagen ethyl methanesulfonate, we were able to isolate three cycloheximide-resistant clones. On the basis of growth kinetics, plating efficiency, and protein synthesis, these clones are 10- to 30-fold more resistant to cycloheximide than the parent cells. Cell-free lysates made from these cells retain 30-65% of their endogenous protein synthesizing ability in the presence of cycloheximide concentrations as high as 300 μg/ml. Protein synthesis in lysates from the parental cells, however, is reduced to about 10% of the control value (i.e., in the absence of drug) at 14 μg of cycloheximide/ml and was completely abolished at 75 μg/ml. These results indicate that cycloheximide resistance in these cells is likely due to an alteration in the protein synthetic machinery. This is the first description of cycloheximide-resistant insect cells, and the best example of cycloheximide resistance in cells originating from a higher eukaryotic organism.
|Original language||English (US)|
|Number of pages||12|
|Journal||Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis|
|State||Published - Oct 1982|
Bibliographical noteFunding Information:
This investigation was supported by Grant AI-11290 from the National Institute of Allergy and Infectious Diseases. A.M.F. was supported by an N.I.H. postdoctoral fellowship. We thank Lily Tao for technical assistance.
Copyright 2014 Elsevier B.V., All rights reserved.