Isolation and characterization of thirteen microsatellite markers for the rabbitfish, siganus fuscescens

Rachel Ravago-Gotanco; Candice Y. Lumibao; Ma Josefa R. Pante

doi:10.1007/s12686-009-9148-y

Isolation and characterization of thirteen microsatellite markers for the rabbitfish, siganus fuscescens

Rachel Ravago-Gotanco, Candice Y. Lumibao, Ma Josefa R. Pante

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The mottled spinefoot, Siganus fuscescens, is an economically important rabbitfish species widely distributed in shallow waters throughout the Indo-Pacific. We describe methods for the identification of thirteen novel microsatellite markers for S. fuscescens using an enrichment protocol. The loci were screened in 126 individuals, revealing moderate to high levels of polymorphism with 5 to 28 alleles per locus and observed heterozygosity ranging from 0.111 to 0.921. No significant deviations from Hardy-Weinberg equilibrium were detected. These polymorphic microsatellites will be useful for studies of genetic diversity, population structure, and demographic connectivity of S. fuscescens.

Original language	English (US)
Pages (from-to)	225-227
Number of pages	3
Journal	Conservation Genetics Resources
Volume	2
Issue number	SUPPL.1
DOIs	https://doi.org/10.1007/s12686-009-9148-y
State	Published - Sep 2010
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments This work was supported by the Global Environment Facility-World Bank Coral Reef Targeted Research Program (CRTR) through the Southeast Asia Center of Excellence. This is contribution number 383 from the University of the Philippines-Marine Science Institute.

Keywords

Connectivity
Microsatellite
Population genetics
Siganus fuscescens

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title = "Isolation and characterization of thirteen microsatellite markers for the rabbitfish, siganus fuscescens",

abstract = "The mottled spinefoot, Siganus fuscescens, is an economically important rabbitfish species widely distributed in shallow waters throughout the Indo-Pacific. We describe methods for the identification of thirteen novel microsatellite markers for S. fuscescens using an enrichment protocol. The loci were screened in 126 individuals, revealing moderate to high levels of polymorphism with 5 to 28 alleles per locus and observed heterozygosity ranging from 0.111 to 0.921. No significant deviations from Hardy-Weinberg equilibrium were detected. These polymorphic microsatellites will be useful for studies of genetic diversity, population structure, and demographic connectivity of S. fuscescens.",

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author = "Rachel Ravago-Gotanco and Lumibao, {Candice Y.} and Pante, {Ma Josefa R.}",

note = "Funding Information: Acknowledgments This work was supported by the Global Environment Facility-World Bank Coral Reef Targeted Research Program (CRTR) through the Southeast Asia Center of Excellence. This is contribution number 383 from the University of the Philippines-Marine Science Institute.",

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doi = "10.1007/s12686-009-9148-y",

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AU - Lumibao, Candice Y.

AU - Pante, Ma Josefa R.

N1 - Funding Information: Acknowledgments This work was supported by the Global Environment Facility-World Bank Coral Reef Targeted Research Program (CRTR) through the Southeast Asia Center of Excellence. This is contribution number 383 from the University of the Philippines-Marine Science Institute.

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N2 - The mottled spinefoot, Siganus fuscescens, is an economically important rabbitfish species widely distributed in shallow waters throughout the Indo-Pacific. We describe methods for the identification of thirteen novel microsatellite markers for S. fuscescens using an enrichment protocol. The loci were screened in 126 individuals, revealing moderate to high levels of polymorphism with 5 to 28 alleles per locus and observed heterozygosity ranging from 0.111 to 0.921. No significant deviations from Hardy-Weinberg equilibrium were detected. These polymorphic microsatellites will be useful for studies of genetic diversity, population structure, and demographic connectivity of S. fuscescens.

AB - The mottled spinefoot, Siganus fuscescens, is an economically important rabbitfish species widely distributed in shallow waters throughout the Indo-Pacific. We describe methods for the identification of thirteen novel microsatellite markers for S. fuscescens using an enrichment protocol. The loci were screened in 126 individuals, revealing moderate to high levels of polymorphism with 5 to 28 alleles per locus and observed heterozygosity ranging from 0.111 to 0.921. No significant deviations from Hardy-Weinberg equilibrium were detected. These polymorphic microsatellites will be useful for studies of genetic diversity, population structure, and demographic connectivity of S. fuscescens.

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Isolation and characterization of thirteen microsatellite markers for the rabbitfish, siganus fuscescens

Abstract

Bibliographical note

Keywords

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