Isolation and identification of antioxidant peptides from commercial soybean-based infant formulas

Patrycja Puchalska, M. Luisa Marina, M. Concepción García

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Soybean-based infant formulas (SBIFs) based on soybean protein isolate (90% of proteins) are an interesting alternative to cow's milk infant formulas. Different works have demonstrated the presence of bioactive peptides in different soybean-based foodstuffs. The aim of this work was the evaluation, for the first time, of antioxidant peptides in five different commercially available SBIFs. Ultrafiltration through 10 kDa molecular weight cut-off filters was the most suitable extraction method. Despite peptide concentrations ranging between 1.19 and 2.27 mg/mL, similar antioxidant capacities were detected in all SBIF extracts. Extracts were further fractionated according to their molecular weight by ultrafiltration, and fractions from 5 to10 kDa, 3 to 5 kDa, and below 3 kDa were obtained. The most active fraction was further fractionated by off-gel isoelectrofocusing and reversed-phase chromatography. Antioxidant fractions were also submitted to simulated gastrointestinal digestion (GI) with pepsin and pancreatin to evaluate their antioxidant capacity after digestion. Peptides were identified by HPLC-ESI-Q-ToF-MS/MS. At least 120 peptides were identified in every antioxidant fraction, with 42 peptides common to all SBIFs.

Original languageEnglish (US)
Pages (from-to)147-154
Number of pages8
JournalFood Chemistry
StatePublished - 2014
Externally publishedYes

Bibliographical note

Funding Information:
Authors thank the Spanish Ministry of Science and Innovation (project CTQ2009-09022, and CTQ 2009-11252) and the Comunidad Autónoma of Madrid (Spain) and European funding from FEDER program (project S2009/AGR-1464, ANALISYC-II). Patrycja Puchalska thanks the University of Alcalá for her pre-doctoral contract.


  • Antioxidant capacity
  • Bioactive peptides
  • Food analysis
  • Q-TOF
  • Soybean based infant formula

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