Isolation and molecular characterization of bovine enteroviruses in Egypt

N. M. Sobhy, S. K. Mor, M. E.M. Mohammed, I. M. Bastawecy, H. M. Fakhry, C. R.B. Youssef, N. Z. Abouzeid, S. M. Goyal

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Enteroviruses belong to the Picornaviridae family and infect a wide range of mammals including cattle. Bovine enterovirus (BEV) has recently been reclassified into E and F serotypes. BEV was first isolated in Egypt in 1966 although it has been known in other countries since the 1950s. In this study, BEV-F2 was isolated from calves with severe diarrhea and the isolated viruses were subjected to molecular characterization. Illumina sequencing of one of the isolates revealed the presence of a complete BEV-F genome sequence. The phylogenetic analysis revealed nucleotide substitutions along the genome in comparison with other known strains of BEV-F (HQ663846, AY508697 and DQ092795). Two primer sets were designed from the 3D and 5'NTR regions and used for the examination of the remaining isolates, which were confirmed to be of the BEV-F2 serotype. The availability of the complete genome sequence of this virus adds to the sequence database of the members of Picornaviridae and should be useful in future molecular studies of BEV.

Original languageEnglish (US)
Pages (from-to)317-321
Number of pages5
JournalVeterinary Journal
Volume206
Issue number3
DOIs
StatePublished - Dec 1 2015

Bibliographical note

Funding Information:
NMS's Fellowship was provided by the Egyptian Ministry of Higher Education and is gratefully acknowledged.

Publisher Copyright:
© 2015 Elsevier Ltd.

Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.

Keywords

  • Bovine enterovirus
  • Cattle
  • Complete sequence
  • Egypt
  • Phylogenetic analysis

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