Isolation of a cDNA for a Growth Factor of Vascular Endothelial Cells from Human Lung Cancer Cells: Its Identity with Insulin‐like Growth Factor II

Koichi Hagiwara, Tatsuo Kobayashi, Masato Tobita, Nobuaki Kikyo, Yoshio Yazaki, Tetsuro Okabe

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We have found growth‐promoting activity for vascular endothelial cells in the conditioned medium of a human lung cancer cell line, T3M‐11. Purification and characterization of the growth‐promoting activity have been carried out using ammonium sulfate precipitation and gel‐exclusion chromatography. The activity migrated as a single peak just after ribonuclease. It did not bind to a heparin affinity column. These results suggest that the activity is not a heparin‐binding growth factor (including fibroblast growth factors) or a vascular endothelial growth factor. To identify the molecule exhibiting the growth‐promoting activity, a cDNA encoding the growth factor was isolated through functional expression cloning in COS‐1 cells from a cDNA library prepared from T3M‐11 cells. The nucleotide sequence encoded by the cDNA proved to be identical with that of insulin‐like growth factor II.

Original languageEnglish (US)
Pages (from-to)202-207
Number of pages6
JournalJapanese Journal of Cancer Research
Volume86
Issue number2
DOIs
StatePublished - Feb 1995

Keywords

  • Expression cloning
  • Growth factor
  • Insulin‐like growth factor II
  • Lung cancer
  • Vascular endothelial cells

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