Abstract
Cell lines were isolated from three stages of embryos of Blattella germanica dissociated with trypsin. The lines have been subcultured 50 to 134 times in 3 years. Line UM-BGE-1 was isolated from germ band embryos at stages of segmentation and limb-bud formation (5 days old). Line UM-BGE-2 was derived from embryos at dorsal closure (7 days old). Line UM-BGE-4 arose from embryos in the germ band and dorsal closure stages (5 and 7 days old); these cells colonize as hollow spheres or vesicles. Line UM-BGE-5, isolated during organogenesis (10 days old), developed into two distinct sublines. Subline α is composed of round cells that do not attach to the flask. Subline β grows as an attached monolayer; the cells can be removed with a saline solution containing 20mM disodium dihydrogen Versenate®. Most of the cells of these lines have the diploid chromosome number (23 or 24) excepting line UM-BGE-1 in which the tetraploid number predominates.
Original language | English (US) |
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Pages (from-to) | 11-17 |
Number of pages | 7 |
Journal | In Vitro |
Volume | 13 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 1977 |
Keywords
- Blattella germanica
- diploid chromosome number
- embryo
- insect
- tetraploid chromosome number