Isolation of cold-adapted nitrate-reducing fungi that have potential to increase nitrate removal in woodchip bioreactors

N. Aldossari; S. Ishii

doi:10.1111/jam.14939

Isolation of cold-adapted nitrate-reducing fungi that have potential to increase nitrate removal in woodchip bioreactors

N. Aldossari, S. Ishii

Soil, Water, and Climate

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Aims: The aim of this study was to obtain cold-adapted denitrifying fungi that could be used for bioaugmentation in woodchip bioreactors to remove nitrate from agricultural subsurface drainage water. Methods and results: We isolated a total of 91 nitrate-reducing fungal strains belonging to Ascomycota and Mucoromycota from agricultural soil and a woodchip bioreactor under relatively cold conditions (5 and 15°C). When these strains were incubated with ¹⁵N-labelled nitrate, ²⁹N₂ was frequently produced, suggesting the occurrence of co-denitrification (microbially mediated nitrosation). Two strains also produced ³⁰N₂, indicating their ability to reduce N₂O. Of the 91 nitrate-reducing fungal strains, fungal nitrite reductase gene (nirK) and cytochrome P450 nitric oxide reductase gene (p450nor) were detected by PCR in 34 (37%) and 11 (12%) strains, respectively. Eight strains possessed both nirK and p450nor, further verifying their denitrification capability. In addition, most strains degraded cellulose under denitrification condition. Conclusions: Diverse nitrate-reducing fungi were isolated from soil and a woodchip bioreactor. These fungi reduced nitrate to gaseous N forms at relatively low temperatures. These cold-adapted, cellulose-degrading and nitrate-reducing fungi could support themselves and other denitrifiers in woodchip bioreactors. Significance and Impact of the Study: The cold-adapted, cellulose-degrading and nitrate-reducing fungi isolated in this study could be useful to enhance nitrate removal in woodchip bioreactors under low-temperature conditions.

Original language	English (US)
Pages (from-to)	197-207
Number of pages	11
Journal	Journal of Applied Microbiology
Volume	131
Issue number	1
DOIs	https://doi.org/10.1111/jam.14939
State	Published - Jul 2021

Bibliographical note

Funding Information:
We thank Dr. Gary Feyereisen at the USDA-ARS for his help with collecting soil and woodchip samples and Dr. Brandy Toner at the University of Minnesota for her helpful comments and encouragement to complete this study. This research was supported by MnDRIVE Initiative of the University of Minnesota. AD was supported by the Saudi Arabian Cultural Mission Scholarship and the Minnesota Mycological Society Scholarship.

Funding Information:
We thank Dr. Gary Feyereisen at the USDA‐ARS for his help with collecting soil and woodchip samples and Dr. Brandy Toner at the University of Minnesota for her helpful comments and encouragement to complete this study. This research was supported by MnDRIVE Initiative of the University of Minnesota. AD was supported by the Saudi Arabian Cultural Mission Scholarship and the Minnesota Mycological Society Scholarship.

Publisher Copyright:
© 2020 The Society for Applied Microbiology

Keywords

cellulose degradation
co-denitrification
denitrification
fungi
soil
woodchip bioreactor

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title = "Isolation of cold-adapted nitrate-reducing fungi that have potential to increase nitrate removal in woodchip bioreactors",

abstract = "Aims: The aim of this study was to obtain cold-adapted denitrifying fungi that could be used for bioaugmentation in woodchip bioreactors to remove nitrate from agricultural subsurface drainage water. Methods and results: We isolated a total of 91 nitrate-reducing fungal strains belonging to Ascomycota and Mucoromycota from agricultural soil and a woodchip bioreactor under relatively cold conditions (5 and 15°C). When these strains were incubated with 15N-labelled nitrate, 29N2 was frequently produced, suggesting the occurrence of co-denitrification (microbially mediated nitrosation). Two strains also produced 30N2, indicating their ability to reduce N2O. Of the 91 nitrate-reducing fungal strains, fungal nitrite reductase gene (nirK) and cytochrome P450 nitric oxide reductase gene (p450nor) were detected by PCR in 34 (37%) and 11 (12%) strains, respectively. Eight strains possessed both nirK and p450nor, further verifying their denitrification capability. In addition, most strains degraded cellulose under denitrification condition. Conclusions: Diverse nitrate-reducing fungi were isolated from soil and a woodchip bioreactor. These fungi reduced nitrate to gaseous N forms at relatively low temperatures. These cold-adapted, cellulose-degrading and nitrate-reducing fungi could support themselves and other denitrifiers in woodchip bioreactors. Significance and Impact of the Study: The cold-adapted, cellulose-degrading and nitrate-reducing fungi isolated in this study could be useful to enhance nitrate removal in woodchip bioreactors under low-temperature conditions.",

keywords = "cellulose degradation, co-denitrification, denitrification, fungi, soil, woodchip bioreactor",

author = "N. Aldossari and S. Ishii",

note = "Funding Information: We thank Dr. Gary Feyereisen at the USDA-ARS for his help with collecting soil and woodchip samples and Dr. Brandy Toner at the University of Minnesota for her helpful comments and encouragement to complete this study. This research was supported by MnDRIVE Initiative of the University of Minnesota. AD was supported by the Saudi Arabian Cultural Mission Scholarship and the Minnesota Mycological Society Scholarship. Funding Information: We thank Dr. Gary Feyereisen at the USDA‐ARS for his help with collecting soil and woodchip samples and Dr. Brandy Toner at the University of Minnesota for her helpful comments and encouragement to complete this study. This research was supported by MnDRIVE Initiative of the University of Minnesota. AD was supported by the Saudi Arabian Cultural Mission Scholarship and the Minnesota Mycological Society Scholarship. Publisher Copyright: {\textcopyright} 2020 The Society for Applied Microbiology",

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doi = "10.1111/jam.14939",

language = "English (US)",

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pages = "197--207",

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T1 - Isolation of cold-adapted nitrate-reducing fungi that have potential to increase nitrate removal in woodchip bioreactors

AU - Aldossari, N.

AU - Ishii, S.

N1 - Funding Information: We thank Dr. Gary Feyereisen at the USDA-ARS for his help with collecting soil and woodchip samples and Dr. Brandy Toner at the University of Minnesota for her helpful comments and encouragement to complete this study. This research was supported by MnDRIVE Initiative of the University of Minnesota. AD was supported by the Saudi Arabian Cultural Mission Scholarship and the Minnesota Mycological Society Scholarship. Funding Information: We thank Dr. Gary Feyereisen at the USDA‐ARS for his help with collecting soil and woodchip samples and Dr. Brandy Toner at the University of Minnesota for her helpful comments and encouragement to complete this study. This research was supported by MnDRIVE Initiative of the University of Minnesota. AD was supported by the Saudi Arabian Cultural Mission Scholarship and the Minnesota Mycological Society Scholarship. Publisher Copyright: © 2020 The Society for Applied Microbiology

PY - 2021/7

Y1 - 2021/7

N2 - Aims: The aim of this study was to obtain cold-adapted denitrifying fungi that could be used for bioaugmentation in woodchip bioreactors to remove nitrate from agricultural subsurface drainage water. Methods and results: We isolated a total of 91 nitrate-reducing fungal strains belonging to Ascomycota and Mucoromycota from agricultural soil and a woodchip bioreactor under relatively cold conditions (5 and 15°C). When these strains were incubated with 15N-labelled nitrate, 29N2 was frequently produced, suggesting the occurrence of co-denitrification (microbially mediated nitrosation). Two strains also produced 30N2, indicating their ability to reduce N2O. Of the 91 nitrate-reducing fungal strains, fungal nitrite reductase gene (nirK) and cytochrome P450 nitric oxide reductase gene (p450nor) were detected by PCR in 34 (37%) and 11 (12%) strains, respectively. Eight strains possessed both nirK and p450nor, further verifying their denitrification capability. In addition, most strains degraded cellulose under denitrification condition. Conclusions: Diverse nitrate-reducing fungi were isolated from soil and a woodchip bioreactor. These fungi reduced nitrate to gaseous N forms at relatively low temperatures. These cold-adapted, cellulose-degrading and nitrate-reducing fungi could support themselves and other denitrifiers in woodchip bioreactors. Significance and Impact of the Study: The cold-adapted, cellulose-degrading and nitrate-reducing fungi isolated in this study could be useful to enhance nitrate removal in woodchip bioreactors under low-temperature conditions.

AB - Aims: The aim of this study was to obtain cold-adapted denitrifying fungi that could be used for bioaugmentation in woodchip bioreactors to remove nitrate from agricultural subsurface drainage water. Methods and results: We isolated a total of 91 nitrate-reducing fungal strains belonging to Ascomycota and Mucoromycota from agricultural soil and a woodchip bioreactor under relatively cold conditions (5 and 15°C). When these strains were incubated with 15N-labelled nitrate, 29N2 was frequently produced, suggesting the occurrence of co-denitrification (microbially mediated nitrosation). Two strains also produced 30N2, indicating their ability to reduce N2O. Of the 91 nitrate-reducing fungal strains, fungal nitrite reductase gene (nirK) and cytochrome P450 nitric oxide reductase gene (p450nor) were detected by PCR in 34 (37%) and 11 (12%) strains, respectively. Eight strains possessed both nirK and p450nor, further verifying their denitrification capability. In addition, most strains degraded cellulose under denitrification condition. Conclusions: Diverse nitrate-reducing fungi were isolated from soil and a woodchip bioreactor. These fungi reduced nitrate to gaseous N forms at relatively low temperatures. These cold-adapted, cellulose-degrading and nitrate-reducing fungi could support themselves and other denitrifiers in woodchip bioreactors. Significance and Impact of the Study: The cold-adapted, cellulose-degrading and nitrate-reducing fungi isolated in this study could be useful to enhance nitrate removal in woodchip bioreactors under low-temperature conditions.

KW - cellulose degradation

KW - co-denitrification

KW - denitrification

KW - fungi

KW - soil

KW - woodchip bioreactor

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Isolation of cold-adapted nitrate-reducing fungi that have potential to increase nitrate removal in woodchip bioreactors

Abstract

Bibliographical note

Keywords

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