Abstract
A micro free flow electrophoresis (μFFE) device was used to select DNA aptamers for human immunoglobulin E (IgE). The continuous nature of μFFE allowed 1.8 × 1014 sequences to be introduced over a period of 30 min, a 300-fold improvement in library size over capillary electrophoresis based selections (CE-SELEX). Four rounds of selection were performed within four days. Aptamers with low nM dissociation constants for IgE were identified after a single round of μFFE selection.
Original language | English (US) |
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Pages (from-to) | 3703-3709 |
Number of pages | 7 |
Journal | Lab on a chip |
Volume | 11 |
Issue number | 21 |
DOIs | |
State | Published - Nov 7 2011 |