Isolation of DNA aptamers using micro free flow electrophoresis

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Abstract

A micro free flow electrophoresis (μFFE) device was used to select DNA aptamers for human immunoglobulin E (IgE). The continuous nature of μFFE allowed 1.8 × 1014 sequences to be introduced over a period of 30 min, a 300-fold improvement in library size over capillary electrophoresis based selections (CE-SELEX). Four rounds of selection were performed within four days. Aptamers with low nM dissociation constants for IgE were identified after a single round of μFFE selection.

Original languageEnglish (US)
Pages (from-to)3703-3709
Number of pages7
JournalLab on a chip
Volume11
Issue number21
DOIs
StatePublished - Nov 7 2011

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