Isolation of HLA locus-specific DNA probes from the 3'-untranslated region

B. H. Koller, B. Sidwell, R. DeMars, H. T. Orr

Research output: Contribution to journalArticlepeer-review

95 Scopus citations

Abstract

When human class I cDNA clones containing coding sequences are used to probe genomic DNA, 15-20 fragments, each containing a complete class I gene or pseudogene, are seen. Identification of which genomic DNA segments encode the HLA-A and -B antigens has to date required transfection of mouse L cells with cloned class I genes or analysis of HLA loss mutants. In this report we show that under high-stringency conditions, probes constructed from the 3'-untranslated region can be used to specifically identify the segments of DNA that encode the HLA-A and -B antigens in the human lymphoblastoid cell line 721. Examination of DNA from unrelated individuals indicates that these probes are locus specific and will permit identifications of HLA-A and -B genes in the population.

Original languageEnglish (US)
Pages (from-to)5175-5178
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number16 I
DOIs
StatePublished - 1984

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