Isolation of nitric oxide synthase in the rat uterus

Yin Jaing, Ashok K. Singh, Mathur S. Kannan, Dana E. Johnson, Ronald L. Shew

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Nitric oxide synthase (NOS) was isolated from the uterus of adult female rats by diethylaminoethyl (DEAE) column and further purified by 2',5'-ADP agarose. The chromatographic properties revealed two isoenzymes, NOS1 and NOS2. The molecular weights of both isoenzymes was approximately 155 Kd by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) which was similar to NOS1 and NOS2 from rat brain. The enzymes required nicotinamide adenine dinucleotide phosphate (NADPH), Ca+2 and calmodulin as cofactors. However, in the absence of calmodulin and/or calcium NOS1 was reduced by approximately 96%, while NOS2 was reduced by approximately 70%. This maximal enzyme activity was similar for brain. These results demonstrate that two isoforms of NOS are present in the rat uterus.

Original languageEnglish (US)
Pages (from-to)1009-1014
Number of pages6
JournalLife Sciences
Volume58
Issue number12
DOIs
StatePublished - Feb 16 1996

Keywords

  • Female reproduction
  • Nitric oxide synthase
  • Sprague-Dawley rat
  • Uterus

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