Isolation of replication-competent molecular clones of visna virus

Katherine A. Staskus; Ernest F. Retzel; Elizabeth D. Lewis; Janet L. Silsby; S. T. Sheila Cyr; Jeffrey M. Rank; Steven W. Wietgrefe; Ashley T. Haase; Ronald Cook; David Fast; Paul T. Geiser; John T. Harty; Selene H. Kong; Carol J. Lahti; Thomas P. Neufeld; Thomas E. Porter; Elizabeth Shoop; Karen R. Zachow

doi:10.1016/0042-6822(91)90488-W

Isolation of replication-competent molecular clones of visna virus

Katherine A. Staskus, Ernest F. Retzel, Elizabeth D. Lewis, Janet L. Silsby, S. T. Sheila Cyr, Jeffrey M. Rank, Steven W. Wietgrefe, Ashley T. Haase, Ronald Cook, David Fast, Paul T. Geiser, John T. Harty, Selene H. Kong, Carol J. Lahti, Thomas P. Neufeld, Thomas E. Porter, Elizabeth Shoop, Karen R. Zachow

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Visna virus is the prototypic member of a subfamily of retroviruses responsible for slow infections of animals and humans. As a part of our investigation of the functions of viral gene products in virus replication, we have isolated three infectious molecular clones and determined the complete nucleotide sequences of two of the clones. We have also characterized the progeny of the biologically cloned viral stocks and of the infectious clones and document considerable heterogeneity in plaque size and antigenic phenotype of the former that is reduced to near homogeneity in the progeny of the infectious clones. It thus should now be possible to trace the emergence of antigenic variants of visna virus as well as ascribe defined functions to structural and regulatory genes of the virus in determining neurovirulence and the slow tempo of infection.

Original language	English (US)
Pages (from-to)	228-240
Number of pages	13
Journal	Virology
Volume	181
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(91)90488-W
State	Published - Mar 1991

Bibliographical note

Funding Information:
We thank Robert Storms and Dennis Anderson for helpful discussions regarding E. co/i hosts and computational analysis, respectively, and the University of Minnesota Department of Microbiology Teaching Lab for technical support during part of the sequencing phase of the project. We also thank Tim Leonard for help in preparation of the figures. This work was supported by a grant from the National Institutes of Health (NS2 1423).

Access

10.1016/0042-6822(91)90488-W

OpenUrl availability

Full text

Cite this

Staskus, K. A., Retzel, E. F., Lewis, E. D., Silsby, J. L., Sheila Cyr, S. T., Rank, J. M., Wietgrefe, S. W., Haase, A. T., Cook, R., Fast, D., Geiser, P. T., Harty, J. T., Kong, S. H., Lahti, C. J., Neufeld, T. P., Porter, T. E., Shoop, E., & Zachow, K. R. (1991). Isolation of replication-competent molecular clones of visna virus. Virology, 181(1), 228-240. https://doi.org/10.1016/0042-6822(91)90488-W

@article{410a5e98a6f34b43b158c4fe64046b04,

title = "Isolation of replication-competent molecular clones of visna virus",

abstract = "Visna virus is the prototypic member of a subfamily of retroviruses responsible for slow infections of animals and humans. As a part of our investigation of the functions of viral gene products in virus replication, we have isolated three infectious molecular clones and determined the complete nucleotide sequences of two of the clones. We have also characterized the progeny of the biologically cloned viral stocks and of the infectious clones and document considerable heterogeneity in plaque size and antigenic phenotype of the former that is reduced to near homogeneity in the progeny of the infectious clones. It thus should now be possible to trace the emergence of antigenic variants of visna virus as well as ascribe defined functions to structural and regulatory genes of the virus in determining neurovirulence and the slow tempo of infection.",

author = "Staskus, {Katherine A.} and Retzel, {Ernest F.} and Lewis, {Elizabeth D.} and Silsby, {Janet L.} and {Sheila Cyr}, {S. T.} and Rank, {Jeffrey M.} and Wietgrefe, {Steven W.} and Haase, {Ashley T.} and Ronald Cook and David Fast and Geiser, {Paul T.} and Harty, {John T.} and Kong, {Selene H.} and Lahti, {Carol J.} and Neufeld, {Thomas P.} and Porter, {Thomas E.} and Elizabeth Shoop and Zachow, {Karen R.}",

note = "Funding Information: We thank Robert Storms and Dennis Anderson for helpful discussions regarding E. co/i hosts and computational analysis, respectively, and the University of Minnesota Department of Microbiology Teaching Lab for technical support during part of the sequencing phase of the project. We also thank Tim Leonard for help in preparation of the figures. This work was supported by a grant from the National Institutes of Health (NS2 1423).",

year = "1991",

month = mar,

doi = "10.1016/0042-6822(91)90488-W",

language = "English (US)",

volume = "181",

pages = "228--240",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Isolation of replication-competent molecular clones of visna virus

AU - Staskus, Katherine A.

AU - Retzel, Ernest F.

AU - Lewis, Elizabeth D.

AU - Silsby, Janet L.

AU - Sheila Cyr, S. T.

AU - Rank, Jeffrey M.

AU - Wietgrefe, Steven W.

AU - Haase, Ashley T.

AU - Cook, Ronald

AU - Fast, David

AU - Geiser, Paul T.

AU - Harty, John T.

AU - Kong, Selene H.

AU - Lahti, Carol J.

AU - Neufeld, Thomas P.

AU - Porter, Thomas E.

AU - Shoop, Elizabeth

AU - Zachow, Karen R.

N1 - Funding Information: We thank Robert Storms and Dennis Anderson for helpful discussions regarding E. co/i hosts and computational analysis, respectively, and the University of Minnesota Department of Microbiology Teaching Lab for technical support during part of the sequencing phase of the project. We also thank Tim Leonard for help in preparation of the figures. This work was supported by a grant from the National Institutes of Health (NS2 1423).

PY - 1991/3

Y1 - 1991/3

N2 - Visna virus is the prototypic member of a subfamily of retroviruses responsible for slow infections of animals and humans. As a part of our investigation of the functions of viral gene products in virus replication, we have isolated three infectious molecular clones and determined the complete nucleotide sequences of two of the clones. We have also characterized the progeny of the biologically cloned viral stocks and of the infectious clones and document considerable heterogeneity in plaque size and antigenic phenotype of the former that is reduced to near homogeneity in the progeny of the infectious clones. It thus should now be possible to trace the emergence of antigenic variants of visna virus as well as ascribe defined functions to structural and regulatory genes of the virus in determining neurovirulence and the slow tempo of infection.

AB - Visna virus is the prototypic member of a subfamily of retroviruses responsible for slow infections of animals and humans. As a part of our investigation of the functions of viral gene products in virus replication, we have isolated three infectious molecular clones and determined the complete nucleotide sequences of two of the clones. We have also characterized the progeny of the biologically cloned viral stocks and of the infectious clones and document considerable heterogeneity in plaque size and antigenic phenotype of the former that is reduced to near homogeneity in the progeny of the infectious clones. It thus should now be possible to trace the emergence of antigenic variants of visna virus as well as ascribe defined functions to structural and regulatory genes of the virus in determining neurovirulence and the slow tempo of infection.

UR - http://www.scopus.com/inward/record.url?scp=0025975535&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025975535&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(91)90488-W

DO - 10.1016/0042-6822(91)90488-W

M3 - Article

C2 - 1847257

AN - SCOPUS:0025975535

SN - 0042-6822

VL - 181

SP - 228

EP - 240

JO - Virology

JF - Virology

IS - 1

ER -

Isolation of replication-competent molecular clones of visna virus

Abstract

Bibliographical note

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this