Itch, an E3 ligase of Oct4, is required for embryonic stem cell self-renewal and pluripotency induction

Bing Liao, Xiaoming Zhong, Huiming Xu, Feng Xiao, Zhuoqing Fang, Junjie Gu, Yue Chen, Yingming Zhao, Ying Jin

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Itch, a C2-WW-HECT domain ubiquitin E3 ligase, plays an important role in various biological processes. However, its role in embryonic stem cells (ESCs) remains unknown. Here, we report that Itch interacts with and targets pluripotency-associated transcription factor Oct4 for ubiquitination. Moreover, Itch enhances Oct4 transcriptional activities and controls Oct4 protein stability dependent on its catalytic activity. Importantly, silencing Itch expression compromises ESC self-renewal capacity and somatic cell reprogramming efficiency. Taken together, our study identifies Itch as a regulator of Oct4 stability and transcriptional activity, establishing a functional link between an E3 ligase and the regulation of pluripotency.

Original languageEnglish (US)
Pages (from-to)1443-1451
Number of pages9
JournalJournal of cellular physiology
Volume228
Issue number7
DOIs
StatePublished - Jul 1 2013

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