TY - JOUR
T1 - It's all about the pentiums
T2 - The use, manipulation, and storage of digital microscopy imaging data for the biological sciences
AU - Hornick, Jessica E.
AU - Hinchcliffe, Edward H.
N1 - Publisher Copyright:
© 2013 Wiley Periodicals, Inc.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Digital microscopy has revolutionized quantitative imaging, with binary-encoded computer files serving to capture and store imaging data sets for analysis. With the ever-present use of computers to generate and store imaging data, it becomes increasingly important to understand how these files are created, and how they can be both used and mis-used. This is a particularly important task for the biologist who may have limited background in computer science. Here we discuss some of the basic aspects of digital data storage and use, including file types, storage media, and the choice between commercial and open-source software. Often, open-source software is written by a user or group of users, and then distributed to the scientific community at large. These can be important tools, but there are some hidden costs to this freeware that we will discuss. We will also compare open-source software to commercial imaging software, which is often written for use by non-computer scientists.
AB - Digital microscopy has revolutionized quantitative imaging, with binary-encoded computer files serving to capture and store imaging data sets for analysis. With the ever-present use of computers to generate and store imaging data, it becomes increasingly important to understand how these files are created, and how they can be both used and mis-used. This is a particularly important task for the biologist who may have limited background in computer science. Here we discuss some of the basic aspects of digital data storage and use, including file types, storage media, and the choice between commercial and open-source software. Often, open-source software is written by a user or group of users, and then distributed to the scientific community at large. These can be important tools, but there are some hidden costs to this freeware that we will discuss. We will also compare open-source software to commercial imaging software, which is often written for use by non-computer scientists.
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U2 - 10.1002/mrd.22294
DO - 10.1002/mrd.22294
M3 - Review article
C2 - 24375801
AN - SCOPUS:84937737560
SN - 1040-452X
VL - 82
SP - 508
EP - 517
JO - Molecular Reproduction and Development
JF - Molecular Reproduction and Development
IS - 7-8
ER -