The kinetics of prourokinase production by human kidney cell line TCL were investigated using microcarriers, roller bottles and a ceramic system. The type of microcarriers used for cell cultivation affects not only growth kinetics but also the duration of post-confluent production period. Different clones of cells derived from the same line can also exhibit different growth kinetics on different types of microcarriers. The effect of serum concentration on the prourokinase production was examined. Using a low serum concentration of 1 % the production of pro-urokinase on microcarriers, roller bottles and a ceramic system was compared. In all three systems the production was sustained over an extended period reaching beyond confluence.
Bibliographical noteFunding Information:
This work was supported in part by grants to W.S.H. from Sandoz AG, Switzerland, Ecolab, Inc., Minnesota, and the National Science Foundation (ECE-8552670). T.Y.T. was a recipient of a fellowship from the Microbial Engineering Program, University of Minnesota. G.Y.J. was a recipient of a fellowship from the government of the People’s Republic of China.
- Ceramic system
- Mammalian cell culture