Kinetochore structure and spindle assembly checkpoint signaling in the budding yeast, Saccharomyces cerevisiae

Duncan J. Clarke, Jeff Bachant

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The Spindle Assembly Checkpoint (SAC) delays the onset of anaphase until every chromosome is properly bioriented at the spindle equator. Mutations in SAC genes have been found in tumors and compromised SAC function can increase the incidence of some carcinomas in mice, providing further links between cancer etiology, chromosome segregation defects and aneuploidy. Here we review recent developments in our understanding of SAC control with particular emphasis on the role of the kinetochore, the nature of the tension sensing mechanism and the possibility that the SAC encompasses more than just stabilization of securin and/or cyclin-B via inhibition of the APC/C to delay anaphase initiation. Our primary emphasis is on the SAC in the budding yeast Saccharomyces cerevisiae. However, relevant findings in other cells are also discussed to highlight the generally conserved nature of SAC signaling mechanisms.

Original languageEnglish (US)
Pages (from-to)6787-6819
Number of pages33
JournalFrontiers in Bioscience
Volume13
Issue number17
StatePublished - May 1 2008

Keywords

  • Anaphase
  • Aneuploidy
  • Chromosome Segregation
  • DNA topoisomerase II
  • Kinetochore
  • Mitotic Checkpoint
  • Review
  • Sister Chromatid Separation
  • Spindle Assembly Checkpoint
  • Tensiometer
  • Tension

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