Abstract
Background: Laminin, a major basement membrane component that has direct contact with pericytes under physiological conditions, actively regulates the proliferation and differentiation/fate determination of pericytes. Recently, two types of pericytes (type I and type II) with different molecular markers and functions have been identified in skeletal muscles. Whether laminin differentially regulates the proliferation and differentiation of these two subpopulations remains unclear. Methods: Wild-type and pericytic laminin-deficient mice under Nestin-GFP background were used to determine if laminin differentially regulates the proliferation and differentiation of type I and type II pericytes. Specifically, type I and type II pericytes were isolated from these mice, and their proliferation and differentiation were examined in vitro. Moreover, in vivo studies were also performed. Results: We demonstrate that, although laminin inhibits the proliferation of both type I and type II pericytes in vitro, loss of laminin predominantly induces proliferation of type II pericytes in vivo. In addition, laminin negatively regulates the adipogenic differentiation of type I pericytes and positively regulates the myogenic differentiation of type II pericytes in vitro. Conclusions: Laminin differentially regulates the proliferation and differentiation of type I and type II pericytes.
Original language | English (US) |
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Article number | 28 |
Pages (from-to) | 1-16 |
Number of pages | 16 |
Journal | Stem Cell Research and Therapy |
Volume | 8 |
Issue number | 1 |
DOIs | |
State | Published - Feb 7 2017 |
Bibliographical note
Funding Information:This work was supported by the Fund-A-Fellow grant from the Myotonic Dystrophy Foundation (MDF-FF-2014-0013) and Scientist Development Grant from American Heart Association (16SDG29320001).
Publisher Copyright:
© 2017 The Author(s).
Keywords
- Differentiation
- Laminin
- Pericytes
- Proliferation