TY - JOUR
T1 - Langerhans cells are not required for epidermal Vγ3 T cell homeostasis and function
AU - Taveirne, Sylvie
AU - de Colvenaer, Veerle
AU - van den Broeck, Tina
AU - van Ammel, Els
AU - Bennett, Clare L.
AU - Taghon, Tom
AU - Vandekerckhove, Bart
AU - Plum, Jean
AU - Clausen, Björn E.
AU - Kaplan, Daniel H.
AU - Leclercq, Georges
PY - 2011/7
Y1 - 2011/7
N2 - This study tested the hypothesis that Vγ3 TCR-bearing T cells are influenced by LCs. Vγ3 T cells and LCs are located in the epidermis of mice. Vγ3 T cells represent the main T cell population in the skin epithelium and play a crucial role in maintaining the skin integrity, whereas LCs are professional APCs. Although Vγ3 T cells and LCs form an interdigitating network in the epidermis, not much is known about their reciprocal influence and/or interdependence. We used two different LC-deficient mouse models, in which LCs are constitutively or inducibly depleted, to investigate the role of LCs in maturation, homeostasis, and function of Vγ3 T cells. We show that Vγ3 T cell numbers are unaltered by LC deficiency, and Vγ3 T cells isolated from LC-deficient mice are phenotypically and upon in vitro stimulation, functionally indistinguishable from Vγ3 T cells isolated from WT mice based on their cytotoxic potential and cytokine production. Additionally, in vivo skinwounding experiments show no major difference in response of Vγ3 T cells to wounding in the absence or presence of LCs. These observations indicate that Vγ3 T cells develop and function independently of LCs.
AB - This study tested the hypothesis that Vγ3 TCR-bearing T cells are influenced by LCs. Vγ3 T cells and LCs are located in the epidermis of mice. Vγ3 T cells represent the main T cell population in the skin epithelium and play a crucial role in maintaining the skin integrity, whereas LCs are professional APCs. Although Vγ3 T cells and LCs form an interdigitating network in the epidermis, not much is known about their reciprocal influence and/or interdependence. We used two different LC-deficient mouse models, in which LCs are constitutively or inducibly depleted, to investigate the role of LCs in maturation, homeostasis, and function of Vγ3 T cells. We show that Vγ3 T cell numbers are unaltered by LC deficiency, and Vγ3 T cells isolated from LC-deficient mice are phenotypically and upon in vitro stimulation, functionally indistinguishable from Vγ3 T cells isolated from WT mice based on their cytotoxic potential and cytokine production. Additionally, in vivo skinwounding experiments show no major difference in response of Vγ3 T cells to wounding in the absence or presence of LCs. These observations indicate that Vγ3 T cells develop and function independently of LCs.
KW - LC deficiency
KW - Langerin DTA mice
KW - Langerin DTR mice
KW - Skin
UR - http://www.scopus.com/inward/record.url?scp=79959832225&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79959832225&partnerID=8YFLogxK
U2 - 10.1189/jlb.1010581
DO - 10.1189/jlb.1010581
M3 - Article
C2 - 21486908
AN - SCOPUS:79959832225
SN - 0741-5400
VL - 90
SP - 61
EP - 68
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 1
ER -