Large-scale gene expression analysis of cholesterol dependence in NS0 cells

Gargi Seth, Robin J. Philp, Claudio D. Denoya, Katherine McGrath, Kim J. Stutzman-Engwall, Miranda Yap, Wei Shou Hu

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

NS0, a nonsecreting mouse myeloma cell, is a major host line used for recombinant antibody production. These cells have a cholesterol-dependent phenotype and rely on an exogenous supply of cholesterol for their survival and growth. To better understand the physiology underlying cholesterol dependence, we compared NS0 cells, cultivated under standard cholesterol-dependent growth conditions (NS0), to cells adapted to cholesterol-independent conditions (NS0 revertant, NS0_r). Large-scale transcriptional analyses were done using the Affy-metrix GeneChip array, MG-U74Av2. The transcripts expressed differentially across the two cell lines were identified. Additionally, proteomic tools were employed to analyze cell lysates from these two cell lines. Cellular proteins from both NS0 and NS0_r were subjected to 2D gel electrophoresis. MALDI-TOF mass spectrometry was performed to determine the identity of the differentially expressed spots. We examined the expression level of mouse genes directly involved in cholesterol biosynthesis, lipid metabolism, and central energy metabolism. Most of these genes were downregulated in the revertant cell type, NS0_r, compared to NS0. Overall, a large number of genes are expressed differentially, indicating that the reversal of cholesterol dependency has a profound effect on cell physiology. It is probable that a single gene mutation, activation, or inactivation is responsible for cholesterol auxotrophy. However, the wide-ranging changes in gene expression point to the distinct possibility of a regulatory event affecting the reversibility of auxotrophy, either directly or indirectly.

Original languageEnglish (US)
Pages (from-to)552-567
Number of pages16
JournalBiotechnology and bioengineering
Volume90
Issue number5
DOIs
StatePublished - Jun 5 2005

Keywords

  • Cholesterol auxotroph
  • DNA microarray
  • Gene expression
  • Mouse myeloma
  • NS0
  • Proteomics

Fingerprint Dive into the research topics of 'Large-scale gene expression analysis of cholesterol dependence in NS0 cells'. Together they form a unique fingerprint.

Cite this