Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

Bi Chang Chen, Wesley R. Legant, Kai Wang, Lin Shao, Daniel E. Milkie, Michael W. Davidson, Chris Janetopoulos, Xufeng S. Wu, John A. Hammer, Zhe Liu, Brian P. English, Yuko Mimori-Kiyosue, Daniel P Romero, Alex T. Ritter, Jennifer Lippincott-Schwartz, Lillian Fritz-Laylin, R. Dyche Mullins, Diana M. Mitchell, Joshua N. Bembenek, Anne Cecile ReymannRalph Böhme, Stephan W. Grill, Jennifer T. Wang, Geraldine Seydoux, U. Serdar Tulu, Daniel P. Kiehart, Eric Betzig

Research output: Contribution to journalArticlepeer-review

804 Scopus citations

Abstract

Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, are too small, or occur too rapidly to see clearly with existing tools.We crafted ultrathin light sheets from two-dimensional optical lattices that allowed us to image three-dimensional (3D) dynamics for hundreds of volumes, often at subsecond intervals, at the diffraction limit and beyond. We applied this to systems spanning four orders of magnitude in space and time, including the diffusion of single transcription factor molecules in stem cell spheroids, the dynamic instability of mitotic microtubules, the immunological synapse, neutrophil motility in a 3D matrix, and embryogenesis in Caenorhabditis elegans and Drosophila melanogaster. The results provide a visceral reminder of the beauty and the complexity of living systems.

Original languageEnglish (US)
Article number1257998
JournalScience
Volume346
Issue number6208
DOIs
StatePublished - Oct 24 2014

Bibliographical note

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© 2014, American Association for the Advancement of Science. All rights reserved.

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