Lc-ms-based metabolomic investigation of chemopreventive phytochemical-elicited metabolic events

Lei Wang, Dan Yao, Chi Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Phytochemicals are under intensive investigation for their potential use as chemopreventive agents in blocking or suppressing carcinogenesis. Metabolic interactions between phytochemical and biological system play an important role in determining the efficacy and toxicity of chemopreventive phytochemicals. However, complexities of phytochemical biotransformation and intermediary metabolism pose challenges for studying phytochemical-elicited metabolic events. Metabolomics has become a highly effective technical platform to detect subtle changes in a complex metabolic system. Here, using green tea polyphenols as an example, we describe a workflow of LC-MS-based metabolomics study, covering the procedures and techniques in sample collection, preparation, LC-MS analysis, data analysis, and interpretation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages77-88
Number of pages12
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1379
ISSN (Print)1064-3745

Keywords

  • Chemoprevention
  • LC-MS
  • Metabolism
  • Metabolomics
  • Phytochemical

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